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GENE EXPRESSION PROFILES IN VIRUS PRODUCING AND LOW/NON VIRUS PRODUCING EBV-BAC CONTAINING CELL LINES

Lucas, Anna (2010) GENE EXPRESSION PROFILES IN VIRUS PRODUCING AND LOW/NON VIRUS PRODUCING EBV-BAC CONTAINING CELL LINES. Master's Thesis, University of Pittsburgh. (Unpublished)

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Abstract

Our lab studies Epstein-Barr virus (EBV); therefore, we need a supply of cells that steadily produce virus for use in our experiments. Currently virus harvesting is unpredictable, as transfection of 293SL cells with a given Bacterial Artificial Chromosome (BAC) may produce cell lines that vary widely in the amount of infectious virus produced, with most lines producing no virus at all. Using quantitative real time PCR we quantified EBV mRNA expression pertaining to 16 specific targets including latent, lytic, and promoter transcripts. This was to determine if there was a correlation between the pattern of virus gene expression and the capacity of a cell line to produce virus. Such a correlation could be used to develop a screening assay predictive of a cell line's potential for virus production. We found that the genes most useful for creating a PCR-based screening assay were the genes belonging to the EBNA3 family. The public health significance of the steady production of Epstein-Barr virus would be that experiments could be conducted on quicker time tables, which in turn may increase the rate of knowledge obtained about EBV.


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Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Lucas, Annarcsonjia@yahoo.com
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairRowe, David Trowe1@pitt.eduROWE1
Committee MemberJenkins, Frankfjenkins@pitt.eduFJENKINS
Committee MemberMartinson, Jeremyjmartins@pitt.eduJMARTINS
Date: 27 January 2010
Date Type: Completion
Defense Date: 14 December 2009
Approval Date: 27 January 2010
Submission Date: 7 December 2009
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Institution: University of Pittsburgh
Schools and Programs: Graduate School of Public Health > Infectious Diseases and Microbiology
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: PCR; transfection; virus production
Other ID: http://etd.library.pitt.edu/ETD/available/etd-12072009-102318/, etd-12072009-102318
Date Deposited: 10 Nov 2011 20:08
Last Modified: 15 Nov 2016 13:53
URI: http://d-scholarship.pitt.edu/id/eprint/10160

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