Lucas, Anna
(2010)
GENE EXPRESSION PROFILES IN VIRUS PRODUCING AND LOW/NON VIRUS PRODUCING EBV-BAC CONTAINING CELL LINES.
Master's Thesis, University of Pittsburgh.
(Unpublished)
Abstract
Our lab studies Epstein-Barr virus (EBV); therefore, we need a supply of cells that steadily produce virus for use in our experiments. Currently virus harvesting is unpredictable, as transfection of 293SL cells with a given Bacterial Artificial Chromosome (BAC) may produce cell lines that vary widely in the amount of infectious virus produced, with most lines producing no virus at all. Using quantitative real time PCR we quantified EBV mRNA expression pertaining to 16 specific targets including latent, lytic, and promoter transcripts. This was to determine if there was a correlation between the pattern of virus gene expression and the capacity of a cell line to produce virus. Such a correlation could be used to develop a screening assay predictive of a cell line's potential for virus production. We found that the genes most useful for creating a PCR-based screening assay were the genes belonging to the EBNA3 family. The public health significance of the steady production of Epstein-Barr virus would be that experiments could be conducted on quicker time tables, which in turn may increase the rate of knowledge obtained about EBV.
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Details
| Item Type: |
University of Pittsburgh ETD
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| Status: |
Unpublished |
| Creators/Authors: |
|
| ETD Committee: |
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| Date: |
27 January 2010 |
| Date Type: |
Completion |
| Defense Date: |
14 December 2009 |
| Approval Date: |
27 January 2010 |
| Submission Date: |
7 December 2009 |
| Access Restriction: |
No restriction; Release the ETD for access worldwide immediately. |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
School of Public Health > Infectious Diseases and Microbiology |
| Degree: |
MS - Master of Science |
| Thesis Type: |
Master's Thesis |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
PCR; transfection; virus production |
| Other ID: |
http://etd.library.pitt.edu/ETD/available/etd-12072009-102318/, etd-12072009-102318 |
| Date Deposited: |
10 Nov 2011 20:08 |
| Last Modified: |
15 Nov 2016 13:53 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/10160 |
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