Tellis, Cari Michelle
(2004)
A Histochemical Analysis of Mitochondrial Abnormalities in the Type I Fibers of Human Posterior Cricoarytenoid Muscle.
Doctoral Dissertation, University of Pittsburgh.
(Unpublished)
Abstract
Mitochondrial deficiencies are present in limb skeletal muscle fibers throughout normal aging and can increase with accumulated exposure to reactive oxide species. Exogenous sources of reactive oxide species include cigarette smoke and other environmental toxins. Intrinsic laryngeal muscles are directly exposed to inhaled toxins which may increase the percentage of cytochrome c oxidase deficient fibers over time. Serial sections of posterior cricoarytenoid and control strap muscle were harvested post laryngectomy from 10 males age 54-78 years old. Cytochrome c oxidase, succinate dehydrogenase, and modified Gomori trichrome stains were used to determine the percentage of type I fibers with cytochrome c oxidase deficiency. Staining with myosin antibodies was used to determine fiber type. The posterior cricoarytenoid muscle contained a significantly higher percentage of type I fibers with cytochrome c oxidase deficiency (p=.002, t = 4.939) compared to the control strap muscle. The percentage of cytochrome c oxidase deficient fibers was also significantly correlated (r = +.851; p < .01) with age in posterior cricoarytenoid muscle. The percentage of type I fibers with cytochrome c oxidase deficiency increases with age in the posterior cricoarytenoid muscle and may be due to the accumulated exposure to reactive oxide species over time.
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Details
| Item Type: |
University of Pittsburgh ETD
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| Status: |
Unpublished |
| Creators/Authors: |
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| ETD Committee: |
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| Date: |
21 December 2004 |
| Date Type: |
Completion |
| Defense Date: |
10 December 2004 |
| Approval Date: |
21 December 2004 |
| Submission Date: |
18 December 2004 |
| Access Restriction: |
No restriction; Release the ETD for access worldwide immediately. |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
School of Health and Rehabilitation Sciences > Communication Science and Disorders |
| Degree: |
PhD - Doctor of Philosophy |
| Thesis Type: |
Doctoral Dissertation |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
Cytochrome c Oxidase; Histochemical; Intrinsic laryngeal muscles; Mitochondrial Abnormalities; Posterior cricoarytenoid |
| Other ID: |
http://etd.library.pitt.edu/ETD/available/etd-12182004-122349/, etd-12182004-122349 |
| Date Deposited: |
10 Nov 2011 20:11 |
| Last Modified: |
19 Dec 2016 14:38 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/10415 |
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