Chu, Hunghao
(2013)
A coacervate-based platform for growth factor delivery.
Doctoral Dissertation, University of Pittsburgh.
(Unpublished)
Abstract
Growth factors participating in a variety of biological processes have great potential in regenerative medicine. However, unprotected growth factors degrade quickly and have little efficacy at tissue repair. Delivery of growth factors with different vehicles has been examined to prolong the half-lives of growth factors and therefore increase its therapeutic efficacy. After decades of research, controlled delivery of growth factor still faces some significant limitations, and none has reached clinical translation.
Heparin, a highly sulfated macromolecule, is used as an anticoagulant clinically. In addition, it has high affinity to a large number of biomolecules, including many growth factors. The interaction between heparin and heparin-binding growth factors is known to adjust their conformation, protect them from proteolytic degradation and regulate their activities. Incorporation of heparin in growth factor delivery is consequently a strategy to potentiate the bioactivity of growth factors. Currently, most approaches used to immobilize heparin on the delivery vehicles rely on covalent modification of heparin that may alter its inherent properties. To maximize the efficacy of heparin, we developed a coacervate-based delivery platform in which heparin is utilized to complex with a polycation without any modification. The polycation neutralizes the negative charges of heparin and precipitates it out of solution. This approach allows spatiotemporal control of the release of heparin-binding growth factors. This dissertation covers the design, production, characterization and application of heparin-based coacervate in controlled release of growth factors.
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Details
| Item Type: |
University of Pittsburgh ETD
|
| Status: |
Unpublished |
| Creators/Authors: |
|
| ETD Committee: |
| Title | Member | Email Address | Pitt Username | ORCID |
|---|
| Committee Chair | Wang, Yadong | | | | | Committee Member | Little, Steven | | | | | Committee Member | Huard, Johnny | | | | | Committee Member | Shroff, Sanjeev | | | |
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| Date: |
16 March 2013 |
| Date Type: |
Publication |
| Defense Date: |
23 November 2011 |
| Approval Date: |
16 March 2012 |
| Submission Date: |
2 December 2011 |
| Access Restriction: |
1 year -- Restrict access to University of Pittsburgh for a period of 1 year. |
| Number of Pages: |
130 |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
Swanson School of Engineering > Bioengineering |
| Degree: |
PhD - Doctor of Philosophy |
| Thesis Type: |
Doctoral Dissertation |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
heparin; growth factor; controlled release; coacervate |
| Date Deposited: |
11 Sep 2013 05:00 |
| Last Modified: |
15 Nov 2016 13:55 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/10653 |
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