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Dengue Virus Infection of B Lymphocytes In Vitro.

Still, Frances (2012) Dengue Virus Infection of B Lymphocytes In Vitro. Master's Thesis, University of Pittsburgh. (Unpublished)

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Abstract

Dengue virus infection of certain subsets of the immune system, especially dendritic cells and macrophages, is well-established in the literature. On these cells, the virus can use DC-SIGN as a receptor. Infection of B lymphocytes is debated, and since B cells upregulate DC-SIGN upon activation, we hypothesize that this receptor may also be used for DENV infection of B cells.
In this thesis, I show that DENV-2 infects primary aB and does not infect resting B cells, while DENV-1 infects neither resting nor aB. DENV-2 infection of aB is DC-SIGN dependent. Infection of Raji/DC-SIGN cells with DENV-2 is DC-SIGN dependent, while Raji/0 infection is observed. Infection is detected via flow cytometry using DENV-specific antibodies: 2H2 for DENV-2 infection, and 3H5.1 for DENV-1 infection.

Statement of Public Health Impact:

Dengue virus infection incidence has increased in recent decades, and poses a particular burden on children in South and Central America. It is known that cells of the immune system are directly involved with disease pathogenesis, and so further investigating infection of specific immune cells is crucial to understanding how best to prevent negative outcomes of DENV infection. B lymphocytes are a key part of the adaptive immune system, and may be infected by
DENV. My thesis directly investigates this question, and also examines if the C-type lectin DC-SIGN is the receptor used by the virus for infection.


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Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Still, Francesfms10@pitt.eduFMS10
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairRappocciolo, Ggiovanna@pitt.eduGIOVANNA
Committee MemberRinaldo, CRrinaldo@pitt.eduRINALDO
Committee MemberEvans, Jevansj2@pitt.eduEVANSJ2
Committee MemberMarques, Emarques@pitt.eduMARQUES
Date: 30 January 2012
Date Type: Publication
Defense Date: 16 December 2011
Approval Date: 30 January 2012
Submission Date: 30 November 2011
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Number of Pages: 48
Institution: University of Pittsburgh
Schools and Programs: Graduate School of Public Health > Infectious Diseases and Microbiology
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: dengue, B lymphocyte, DC-SIGN
Date Deposited: 30 Jan 2012 18:38
Last Modified: 15 Nov 2016 13:55
URI: http://d-scholarship.pitt.edu/id/eprint/10868

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