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Nitric oxide metabolism in canine sepsis: Relation to regional blood flow

Tanigawa, K and Bellomo, R and Kellum, JA and Kim, YM and Zar, H and Lancaster, JR and Pinsky, MR and Ondulick, B (1999) Nitric oxide metabolism in canine sepsis: Relation to regional blood flow. Journal of Critical Care, 14 (4). 186 - 190. ISSN 0883-9441

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Purpose: To investigate the role of nitric oxide (NO) in early endotoxemia on the systemic and regional blood flow by measuring the plasma nitrite/nitrate (NOx) and blood nitrosyl-hemoglobin (NO-Hb) levels. Materials and Methods: This was a prospective, controlled, experimental study conducted in an animal research laboratory on 15 male mongrel dogs. Escherichia coli endotoxin (1 mg/kg) was injected intravenously. Results: Hepatic, renal, and iliac blood flow and cardiac output (CO) were measured before and 15, 30, 45, 90 and 180 minutes after injection of Escherichia coli endotoxin (1 mg/kg) (n = 6). NOx efflux from the organs was calculated by measuring plasma NOx levels. The arterial blood levels of NO-Hb were also measured (n = 4). As control studies, blood samples from dogs (n = 5) without exposure to endotoxin were assayed at 180 minutes for NOx and NO-Hb. Following endotoxin injection, mean arterial pressure decreased and reached its lowest value at 90 minutes (baseline vs. 90 minutes: 119.1 ± 5.8 vs. 82.5 ± 16.7 mm Hg, P< .0001). Hepatic artery blood flow increased significantly (baseline vs. 180 minutes: 23.6 ± 12.0 vs. 170.0 ± 68.4 mL/ min, P < .0001). There were no significant changes in plasma levels of NOx, uptake or release of NOx across the measured vascular beds, NO-Hb levels at any time point. In the portal system, the portal vein flow correlated with NOx release (R = 0.69, P < .0001). Conclusion: in the early phase of endotoxemia in the dog, the significant reduction in systemic vascular resistance and hepatic arterial resistance are not associated with any measurable NOx release in the systemic circulation or the liver.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Tanigawa, K
Bellomo, R
Kellum, JAkellum@pitt.eduKELLUM0000-0003-1995-2653
Kim, YM
Zar, H
Lancaster, JR
Pinsky, MRpinsky@pitt.eduPINSKY0000-0001-6166-700X
Ondulick, B
Date: 1 January 1999
Date Type: Publication
Journal or Publication Title: Journal of Critical Care
Volume: 14
Number: 4
Page Range: 186 - 190
DOI or Unique Handle: 10.1016/s0883-9441(99)90033-3
Schools and Programs: School of Medicine > Critical Care Medicine
Refereed: Yes
ISSN: 0883-9441
PubMed ID: 10622753
Date Deposited: 22 Mar 2012 20:49
Last Modified: 30 Jan 2020 16:55


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