Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

Mitochondrial membrane potential and apoptosis peripheral blood monocytes in severe human sepsis

Adrie, C and Bachelet, M and Vayssier-Taussat, M and Russo-Marie, F and Bouchaert, I and Adib-Conquy, M and Cavaillon, JM and Pinsky, MR and Dhainaut, JF and Polla, BS (2001) Mitochondrial membrane potential and apoptosis peripheral blood monocytes in severe human sepsis. American Journal of Respiratory and Critical Care Medicine, 164 (3). 389 - 395. ISSN 1073-449X

[img] Plain Text (licence)
Available under License : See the attached license file.

Download (1kB)


Reduced mitochondrial membrane potential (Δψm), which is considered as an initial and irreversible step towards apoptosis, as well as cell death regulating proteins, such as Fas, Hsp70, or Bcl-2, may play an important role in sepsis. We studied the relationship between sepsis severity and peripheral blood monocyte ΔΨm, cell death (necrosis and apoptosis), soluble Fas ligand, Hsp70, and Bcl-2 expression over time in 18 patients with sepsis, and compared these data with those of a group of 17 healthy control subjects. All measurements were performed within 3 d of the onset of severe sepsis (T1), then 7 to 10 d later (T2), and finally at hospital discharge (T3). ΔΨm was expressed as the percent monocytes with altered ΔΨm (%ΔΨm). Patients with sepsis had greater %ΔΨm at T1 and T2 but not at T3 (14.6 ± 2.6% and 15.9 ± 2%, respectively, versus control 6.6 ± 0.2%, p < 0.01). Septic patients exhibited greater cell death in their monocytes and had greater Hsp70 expression only at T1. Bcl-2 levels were similar in septic and control subjects. Comparing survivors with non-survivors of sepsis, non-survivors had a greater %ΔΨm at T1 (26.4 ± 5.3% versus 10.1 ± 2.7%, p < 0.01) and a significant decrease in Bcl-2 expression, whereas no difference was found in Hsp70 levels. These results indicate that mitochondrial dysfunction and subsequent cell death occur in severe sepsis and suggest that %ΔΨm is a marker of severity in human sepsis.


Social Networking:
Share |


Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Adrie, C
Bachelet, M
Vayssier-Taussat, M
Russo-Marie, F
Bouchaert, I
Adib-Conquy, M
Cavaillon, JM
Pinsky, MRpinsky@pitt.eduPINSKY0000-0001-6166-700X
Dhainaut, JF
Polla, BS
Date: 1 August 2001
Date Type: Publication
Journal or Publication Title: American Journal of Respiratory and Critical Care Medicine
Volume: 164
Number: 3
Page Range: 389 - 395
DOI or Unique Handle: 10.1164/ajrccm.164.3.2009088
Schools and Programs: School of Medicine > Critical Care Medicine
Refereed: Yes
ISSN: 1073-449X
PubMed ID: 11500338
Date Deposited: 20 Mar 2012 16:08
Last Modified: 30 Jan 2020 16:56


Monthly Views for the past 3 years

Plum Analytics

Actions (login required)

View Item View Item