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Control of phage Bxb1 excision by a novel recombination directionality factor

Ghosh, P and Wasil, LR and Hatfull, GF (2006) Control of phage Bxb1 excision by a novel recombination directionality factor. PLoS Biology, 4 (6). 0964 - 0974. ISSN 1545-7885

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Abstract

Mycobacteriophage Bxb1 integrates its DNA at the attB site of the Mycobacterium smegmatis genome using the viral attP site and a phage-encoded integrase generating the recombinant junctions attL and attR. The Bxb1 integrase is a member of the serine recombinase family of site-specific recombination proteins and utilizes small (<50 base pair) substrates for recombination, promoting strand exchange without the necessity for complex higher order macromolecular architectures. To elucidate the regulatory mechanism for the integration and excision reactions, we have identified a Bxb1-encoded recombination directionality factor (RDF), the product of gene 47. Bxb1 gp47 is an unusual RDF in that it is relatively large (∼28 kDa), unrelated to all other RDFs, and presumably performs dual functions since it is well conserved in mycobacteriophages that utilize unrelated integration systems. Furthermore, unlike other RDFs, Bxb1 gp47 does not bind DNA and functions solely through direct interaction with integrase-DNA complexes. The nature and consequences of this interaction depend on the specific DNA substrate to which integrase is bound, generating electrophoretically stable tertiary complexes with either attB or attP that are unable to undergo integrative recombination, and weakly bound, electrophoretically unstable complexes with either attL or attR that gain full potential for excisive recombination. © 2006 Ghosh et al.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Ghosh, P
Wasil, LRLRW31@pitt.eduLRW31
Hatfull, GFgfh@pitt.eduGFH
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorMichel, BenedicteUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 26 June 2006
Date Type: Publication
Journal or Publication Title: PLoS Biology
Volume: 4
Number: 6
Page Range: 0964 - 0974
DOI or Unique Handle: 10.1371/journal.pbio.0040186
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 1545-7885
PubMed ID: 16719562
Date Deposited: 11 Jul 2012 18:02
Last Modified: 04 Feb 2019 15:55
URI: http://d-scholarship.pitt.edu/id/eprint/12849

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