Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

DC-SIGN on B lymphocytes is required for transmission of HIV-1 to T lymphocytes.

Rappocciolo, G and Piazza, P and Fuller, CL and Reinhart, TA and Watkins, SC and Rowe, DT and Jais, M and Gupta, P and Rinaldo, CR (2006) DC-SIGN on B lymphocytes is required for transmission of HIV-1 to T lymphocytes. PLoS pathogens, 2 (7).

[img]
Preview
PDF
Published Version
Available under License : See the attached license file.

Download (685kB) | Preview
[img] Plain Text (licence)
Available under License : See the attached license file.

Download (1kB)

Abstract

Infection of T cells by HIV-1 can occur through binding of virus to dendritic cell (DC)-specific ICAM-3 grabbing nonintegrin (DC-SIGN) on dendritic cells and transfer of virus to CD4+ T cells. Here we show that a subset of B cells in the blood and tonsils of normal donors expressed DC-SIGN, and that this increased after stimulation in vitro with interleukin 4 and CD40 ligand, with enhanced expression of activation and co-stimulatory molecules CD23, CD58, CD80, and CD86, and CD22. The activated B cells captured and internalized X4 and R5 tropic strains of HIV-1, and mediated trans infection of T cells. Pretreatment of the B cells with anti-DC-SIGN monoclonal antibody blocked trans infection of T cells by both strains of HIV-1. These results indicate that DC-SIGN serves as a portal on B cells for HIV-1 infection of T cells in trans. Transmission of HIV-1 from B cells to T cells through this DC-SIGN pathway could be important in the pathogenesis of HIV-1 infection.


Share

Citation/Export:
Social Networking:
Share |

Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Rappocciolo, Ggiovanna@pitt.eduGIOVANNA
Piazza, Ppaolo@pitt.eduPAOLO
Fuller, CL
Reinhart, TAreinhar@pitt.eduREINHAR
Watkins, SCsimon.watkins@pitt.eduSWATKINS
Rowe, DTrowe1@pitt.eduROWE1
Jais, M
Gupta, Ppgupta1@pitt.eduPGUPTA1
Rinaldo, CRRINALDO@pitt.eduRINALDO
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorKoup, RichardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 1 July 2006
Date Type: Publication
Journal or Publication Title: PLoS pathogens
Volume: 2
Number: 7
DOI or Unique Handle: 10.1371/journal.ppat.0020070
Schools and Programs: Graduate School of Public Health > Infectious Diseases and Microbiology
School of Medicine > Cell Biology and Molecular Physiology
Refereed: Yes
PubMed ID: 16839201
Date Deposited: 11 Jul 2012 17:30
Last Modified: 30 Oct 2018 14:02
URI: http://d-scholarship.pitt.edu/id/eprint/12851

Metrics

Monthly Views for the past 3 years

Plum Analytics

Altmetric.com


Actions (login required)

View Item View Item