Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

Potent nonnucleoside reverse transcriptase inhibitors target HIV-1 Gag-Pol

Figueiredo, A and Moore, KL and Mak, J and Sluis-Cremer, N and De Bethune, MP and Tachedjian, G (2006) Potent nonnucleoside reverse transcriptase inhibitors target HIV-1 Gag-Pol. PLoS Pathogens, 2 (11). 1051 - 1059. ISSN 1553-7366

Published Version
Available under License : See the attached license file.

Download (752kB) | Preview
[img] Plain Text (licence)
Available under License : See the attached license file.

Download (1kB)


Nonnucleoside reverse transcriptase inhibitors (NNRTIs) target HIV-1 reverse transcriptase (RT) by binding to a pocket in RT that is close to, but distinct, from the DNA polymerase active site and prevent the synthesis of viral cDNA. NNRTIs, in particular, those that are potent inhibitors of RT polymerase activity, can also act as chemical enhancers of the enzyme's inter-subunit interactions. However, the consequences of this chemical enhancement effect on HIV-1 replication are not understood. Here, we show that the potent NNRTIs efavirenz, TMC120, and TMC125, but not nevirapine or delavirdine, inhibit the late stages of HIV-1 replication. These potent NNRTIs enhanced the intracellular processing of Gag and Gag-Pol polyproteins, and this was associated with a decrease in viral particle production from HIV-1-transfected cells. The increased polyprotein processing is consistent with premature activation of the HIV-1 protease by NNRTI-enhanced Gag-Pol multimerization through the embedded RT sequence. These findings support the view that Gag-Pol multimerization is an important step in viral assembly and demonstrate that regulation of Gag-Pol/Gag-Pol interactions is a novel target for small molecule inhibitors of HIV-1 production. Furthermore, these drugs can serve as useful probes to further understand processes involved in HIV-1 particle assembly and maturation. © 2006 Figueiredo et al.


Social Networking:
Share |


Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Figueiredo, A
Moore, KL
Mak, J
Sluis-Cremer, Nnps2@pitt.eduNPS2
De Bethune, MP
Tachedjian, G
ContributionContributors NameEmailPitt UsernameORCID
Date: 1 November 2006
Date Type: Publication
Journal or Publication Title: PLoS Pathogens
Volume: 2
Number: 11
Page Range: 1051 - 1059
DOI or Unique Handle: 10.1371/journal.ppat.0020119
Refereed: Yes
ISSN: 1553-7366
PubMed ID: 17096588
Date Deposited: 11 Jul 2012 17:28
Last Modified: 31 Jul 2020 17:01


Monthly Views for the past 3 years

Plum Analytics

Actions (login required)

View Item View Item