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K13 blocks KSHV lytic replication and deregulates vIL6 nad hIL6 expression: A model of lytic replication induced clonal selection in viral oncogenesis

Zhao, J and Punj, V and Matta, H and Mazzacurati, L and Schamus, S and Yang, Y and Yang, T and Hong, Y and Chaudhary, PM (2007) K13 blocks KSHV lytic replication and deregulates vIL6 nad hIL6 expression: A model of lytic replication induced clonal selection in viral oncogenesis. PLoS ONE, 2 (10).

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Abstract

Background. Accumulating evidence suggests that dysregulated expression of lytic genes plays an important role in KSHV (Kaposi's sarcoma associated herpesvirus) tumorigenesis. However, the molecular events leading to the dysregulation of KSHV lytic gene expression program are incompletely understood. Methodoloxy/Principal Findings. We have studied the effect of KSHV-encoded latent protein vFLIP K13, a potent activator of the NF-κB pathway, on lytic reactivation of the virus. We demonstrate that K13 antagonizes RTA, the KSHV lytic-regulator, and effectively blocks the expression of lytic proteins, production of infectious virions and death of the infected cells. Induction of lytic replication selects for clones with increased K13 expression and NF-κB activity, while siRNA-mediated silencing of K13 induces the expression of lytic genes. However, the suppressive effect of K13 on RTA-induced lytic genes is not uniform and it falls to block RTA-induced viral IL6 secretion and cooperates with RTA to enhance cellular IL-6 production, thereby dysregulating the lytic gene expression program. Conclusions/Significance. Our results support a model in which ongoing KSHV, lytic replication selects for clones with progressively higher levels of K13 expression and NF-κB activity, which in turn drive KSHV tumorigenesis by not only directly stimulating cellular survival and proliferation, but also indirectly by dysregulating the viral lytic gene program and allowing non-lytic production of growth-promoting viral and cellular genes. Lytic Replication-Induced Clonal Selection (LyRICS) may represent a general mechanism in viral oncogenesis. 2007 Zhao et al.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Zhao, J
Punj, V
Matta, H
Mazzacurati, L
Schamus, S
Yang, Y
Yang, T
Hong, Y
Chaudhary, PM
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorDong-Yan, JinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Centers: Other Centers, Institutes, or Units > Hillman Cancer Center
Date: 24 October 2007
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 2
Number: 10
DOI or Unique Handle: 10.1371/journal.pone.0001067
Refereed: Yes
PubMed ID: 17957251
Date Deposited: 18 Jul 2012 21:06
Last Modified: 05 Feb 2019 07:55
URI: http://d-scholarship.pitt.edu/id/eprint/12921

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