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Phosphatidylserine targets single-walled carbon nanotubes to professional phagocytes in vitro and in vivo

Konduru, NV and Tyurina, YY and Feng, W and Basova, LV and Belikova, NA and Bayir, H and Clark, K and Rubin, M and Stolz, D and Vallhov, H and Scheynius, A and Witasp, E and Fadeel, B and Kichambare, PD and Star, A and Kisin, ER and Murray, AR and Shvedova, AA and Kagan, VE (2009) Phosphatidylserine targets single-walled carbon nanotubes to professional phagocytes in vitro and in vivo. PLoS ONE, 4 (2).

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Broad applications of single-walled carbon nanotubes (SWCNT) dictate the necessity to better understand their health effects. Poor recognition of non-functionalized SWCNT by phagocytes is prohibitive towards controlling their biological-action. We report that SWCNT coating with a phospholipid "eat-me" signal, phosphatidylserine (PS), makes them recognizable in vitro by different phagocytic cells - murine RAW264.7 macrophages, primary monocyte-derived human macrophages, dendritic cells, and rat brain microglia. Macrophage uptake of PS-coated nanotubes was suppressed by the PS-binding protein, Annexin V, and endocytosis inhibitors, and changed the pattern of pro- and anti-inflammatory cytokine secretion. Loading of PS-coated SWCNT with pro-apoptotic cargo (cytochrome c) allowed for the targeted killing of RAW264.7 macrophages. In vivo aspiration of PS-coated SWCNT stimulated their uptake by lung alveolar macrophages in mice. Thus, PS-coating can be utilized for targeted delivery of SWCNT with specified cargoes into professional phagocytes, hence for therapeutic regulation of specific populations of immune-competent cells.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Konduru, NV
Tyurina, YYyyt1@pitt.eduYYT1
Feng, W
Basova, LV
Belikova, NA
Bayir, Hhub22@pitt.eduHUB220000-0003-2361-4120
Clark, Kkac220@pitt.eduKAC220
Rubin, M
Stolz, Ddonna.stolz@pitt.eduDSTOLZ
Vallhov, H
Scheynius, A
Witasp, E
Fadeel, B
Kichambare, PD
Star, Aastar@pitt.eduASTAR
Kisin, ER
Murray, AR
Shvedova, AA
Kagan, VEkagan@pitt.eduKAGAN
ContributionContributors NameEmailPitt UsernameORCID
Date: 9 February 2009
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 4
Number: 2
DOI or Unique Handle: 10.1371/journal.pone.0004398
Schools and Programs: School of Public Health > Environmental and Occupational Health
Dietrich School of Arts and Sciences > Chemistry
School of Medicine > Cell Biology and Molecular Physiology
Refereed: Yes
PubMed Central ID: PMC2634966
PubMed ID: 19198650
Date Deposited: 24 Jul 2012 18:58
Last Modified: 19 Aug 2020 20:55


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