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Genetic characterization of type A enterotoxigenic Clostridium perfringens strains

Deguchi, A and Miyamoto, K and Kuwahara, T and Miki, Y and Kaneko, I and Li, J and McClane, BA and Akimoto, S (2009) Genetic characterization of type A enterotoxigenic Clostridium perfringens strains. PLoS ONE, 4 (5).

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Abstract

Clostridium perfringens type A, is both a ubiquitous environmental bacterium and a major cause of human gastrointestinal disease, which usually involves strains producing C. perfringens enterotoxin (CPE). The gene (cpe) encoding this toxin can be carried on the chromosome or a large plasmid. Interestingly, strains carrying cpe on the chromosome and strains carrying cpe on a plasmid often exhibit different biological characteristics, such as resistance properties against heat. In this study, we investigated the genetic properties of C. perfringens by PCR-surveying 21 housekeeping genes and genes on representative plasmids and then confirmed those results by Southern blot assay (SB) of five genes. Furthermore, sequencing analysis of eight housekeeping genes and multilocus sequence typing (MLST) analysis were also performed. Fifty-eight C. perfringens strains were examined, including isolates from: food poisoning cases, human gastrointestinal disease cases, foods in Japan or the USA, or feces of healthy humans. In the PCR survey, eight of eleven housekeeping genes amplified positive reactions in all strains tested. However, by PCR survey and SB assay, one representative virulence gene, pfoA, was not detected in any strains carrying cpe on the chromosome. Genes involved in conjugative transfer of the cpe plasmid were also absent from almost all chromosomal cpe strains. MLST showed that, regardless of their geographic origin, date of isolation, or isolation source, chromosomal cpe isolates, i) assemble into one definitive cluster ii) lack pfoA and iii) lack a plasmid related to the cpe plasmid. Similarly, independent of their origin, strains carrying a cpe plasmid also appear to be related, but are more variable than chromosomal cpe strains, possibly because of the instability of cpe-borne plasmid(s) and/or the conjugative transfer of cpe-plasmid(s) into unrelated C. perfringens strains. © 2009 Deguchi et al.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Deguchi, A
Miyamoto, K
Kuwahara, T
Miki, Y
Kaneko, I
Li, Jjihongli@pitt.eduJIHONGLI
McClane, BAbamcc@pitt.eduBAMCC
Akimoto, S
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorBereswill, StefanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 19 May 2009
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 4
Number: 5
DOI or Unique Handle: 10.1371/journal.pone.0005598
Refereed: Yes
PubMed Central ID: PMC2682570
PubMed ID: 19479065
Date Deposited: 25 Jul 2012 14:12
Last Modified: 02 Feb 2019 16:58
URI: http://d-scholarship.pitt.edu/id/eprint/13141

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