Expanding the diversity of mycobacteriophages: Insights into genome architecture and evolution

Pope, WH and Jacobs-Sera, D and Russel, DA and Peebles, CL and Al-Atrache, Z and Alcoser, TA and Alexander, LM and Alfano, MB and Alford, ST and Amy, NE and Anderson, MD and Anderson, AG and Ang, AAS and Manuel, A and Barber, AJ and Barker, LP and Barrett, JM and Barshop, WD and Bauerle, CM and Bayles, IM and Belfield, KL and Best, AA and Agustin, B and Bowman, CA and Boyer, CA and Bradley, KW and Bradley, VA and Broadway, LN and Budwal, K and Busby, KN and Campbell, IW and Campbell, AM and Carey, A and Caruso, SM and Chew, RD and Cockburn, CL and Cohen, LB and Corajod, JM and Cresawn, SG and Davis, KR and Deng, L and Denver, DR and Dixon, BR and Ekram, S and Elgin, SCR and Engelsen, AE and English, BEV and Erb, ML and Estrada, C and Filliger, LZ and Findley, AM and Forbes, L and Forsyth, MH and Fox, TM and Fritz, MJ and Garcia, R and George, ZD and Georges, AE and Gissendanner, CR and Goff, S and Goldstein, R and Gordon, KC and Green, RD and Guerra, SL and Guiney-Olsen, KR and Guiza, BG and Haghighat, L and Hagopian, GV and Harmon, CJ and Harmson, JS and Hartzog, GA and Harvey, SE and He, S and He, KJ and Healy, KE and Higinbotham, ER and Hildebrandt, EN and Ho, JH and Hogan, GM and Hohenstein, VG and Holz, NA and Huang, VJ and Hufford, EL and Hynes, PM and Jackson, AS and Jansen, EC and Jarvik, J and Jasinto, PG and Jordan, TC and Kasza, T and Katelyn, MA and Kelsey, JS and Kerrigan, LA and Khaw, D and Kim, J and Knutter, JZ and Ko, CC and Larkin, GV and Laroche, JR and Latif, A (2011) Expanding the diversity of mycobacteriophages: Insights into genome architecture and evolution. PLoS ONE, 6 (1).

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Abstract

Mycobacteriophages are viruses that infect mycobacterial hosts such as Mycobacterium smegmatis and Mycobacterium tuberculosis. All mycobacteriophages characterized to date are dsDNA tailed phages, and have either siphoviral or myoviral morphotypes. However, their genetic diversity is considerable, and although sixty-two genomes have been sequenced and comparatively analyzed, these likely represent only a small portion of the diversity of the mycobacteriophage population at large. Here we report the isolation, sequencing and comparative genomic analysis of 18 new mycobacteriophages isolated from geographically distinct locations within the United States. Although no clear correlation between location and genome type can be discerned, these genomes expand our knowledge of mycobacteriophage diversity and enhance our understanding of the roles of mobile elements in viral evolution. Expansion of the number of mycobacteriophages grouped within Cluster A provides insights into the basis of immune specificity in these temperate phages, and we also describe a novel example of apparent immunity theft. The isolation and genomic analysis of bacteriophages by freshman college students provides an example of an authentic research experience for novice scientists. © 2011 Hatfull et al.

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Details

Item Type:	Article
Status:	Published
Creators/Authors:	Creators Email Pitt Username ORCID Pope, WH Jacobs-Sera, D djs@pitt.edu DJS 0000-0001-7838-3257 Russel, DA Peebles, CL cpeebles@pitt.edu CPEEBLES 0000-0001-7938-3312 Al-Atrache, Z Alcoser, TA Alexander, LM Alfano, MB Alford, ST Amy, NE Anderson, MD Anderson, AG Ang, AAS Manuel, A Barber, AJ Barker, LP Barrett, JM Barshop, WD Bauerle, CM Bayles, IM Belfield, KL Best, AA Agustin, B Bowman, CA Boyer, CA Bradley, KW Bradley, VA Broadway, LN Budwal, K Busby, KN Campbell, IW Campbell, AM Carey, A Caruso, SM Chew, RD Cockburn, CL Cohen, LB Corajod, JM Cresawn, SG Davis, KR Deng, L Denver, DR Dixon, BR Ekram, S Elgin, SCR Engelsen, AE English, BEV Erb, ML Estrada, C Filliger, LZ Findley, AM Forbes, L Forsyth, MH Fox, TM Fritz, MJ Garcia, R George, ZD Georges, AE Gissendanner, CR Goff, S Goldstein, R Gordon, KC Green, RD Guerra, SL Guiney-Olsen, KR Guiza, BG Haghighat, L Hagopian, GV Harmon, CJ Harmson, JS Hartzog, GA Harvey, SE He, S He, KJ Healy, KE Higinbotham, ER Hildebrandt, EN Ho, JH Hogan, GM Hohenstein, VG Holz, NA Huang, VJ Hufford, EL Hynes, PM Jackson, AS Jansen, EC Jarvik, J Jasinto, PG Jordan, TC Kasza, T Katelyn, MA Kelsey, JS Kerrigan, LA Khaw, D Kim, J Knutter, JZ Ko, CC Larkin, GV Laroche, JR Latif, A
Date:	7 February 2011
Date Type:	Publication
Journal or Publication Title:	PLoS ONE
Volume:	6
Number:	1
DOI or Unique Handle:	10.1371/journal.pone.0016329
Schools and Programs:	Dietrich School of Arts and Sciences > Biological Sciences
Refereed:	Yes
MeSH Headings:	Base Sequence; Biological Evolution; DNA, Viral--genetics; Genetic Variation; Genome, Viral--genetics; Geography; Mycobacteriophages--genetics; Mycobacteriophages--immunology; Mycobacteriophages--isolation & purification; Sequence Analysis, DNA; United States
Other ID:	NLM PMC3029335
PubMed Central ID:	PMC3029335
PubMed ID:	21298013
Date Deposited:	03 Aug 2012 16:05
Last Modified:	31 Jul 2020 15:57
URI:	http://d-scholarship.pitt.edu/id/eprint/13258

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