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HIV-1 infection of DC: Evidence for the acquisition of virus particles from infected T cells by antigen uptake mechanism

Venkatachari, NJ and Alber, S and Watkins, SC and Ayyavoo, V (2009) HIV-1 infection of DC: Evidence for the acquisition of virus particles from infected T cells by antigen uptake mechanism. PLoS ONE, 4 (10).

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Abstract

Dendritic cells (DC) play a pivotal role in transmission and dissemination of HIV-1. Earlier studies reported that DC present at the site of infection trap virus particles via DC-SIGN and transfer the virus to the interacting naïve T cells. This prompted us to ask the question whether DC could acquire virus from infected T cells during DC-T cell interaction. To address this, we investigated the likely transfer of virus from HIV-1 infected T cells to DC and the underlying mechanisms involved. Results indicate that DC acquire virus from infected T cells via antigen uptake mechanism and this results in infection of DC with expression of proteins directed by viral DNA. Further studies with HIV-1 lacking the Env protein also resulted in infection of DC. The use of antibodies against DC-SIGN and DC-SIGN-R ruled out a role for receptor in the infection of DC. Additional data show that DC infection is directly correlated with the ability of DC to take up antigen from infected T cells. Overall, these studies provide evidence to suggest that HIV-1, besides infecting immune cells, also utilizes immunological mechanism(s) to acquire and disseminate virus. © 2009 Venkatachari et al.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Venkatachari, NJ
Alber, S
Watkins, SCsimon.watkins@pitt.eduSWATKINS
Ayyavoo, Vvelpandi@pitt.eduVELPANDI
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorWang, Fu-ShengUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 15 October 2009
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 4
Number: 10
DOI or Unique Handle: 10.1371/journal.pone.0007470
Schools and Programs: Graduate School of Public Health > Infectious Diseases and Microbiology
School of Medicine > Cell Biology and Molecular Physiology
Refereed: Yes
MeSH Headings: CD4-Positive T-Lymphocytes--virology; Cell Adhesion Molecules--metabolism; Dendritic Cells--virology; Green Fluorescent Proteins--chemistry; HIV Infections--metabolism; HIV Infections--virology; HIV-1--metabolism; Humans; Jurkat Cells; Lectins, C-Type--metabolism; Leukocytes, Mononuclear--cytology; Mannose-Binding Lectins--metabolism; Microscopy, Fluorescence--methods; Phenotype; Plasmids--metabolism; Receptors, Cell Surface--metabolism; T-Lymphocytes--metabolism; T-Lymphocytes--virology
Other ID: NLM PMC2759578
PubMed Central ID: PMC2759578
PubMed ID: 19829715
Date Deposited: 03 Aug 2012 16:22
Last Modified: 02 Feb 2019 15:55
URI: http://d-scholarship.pitt.edu/id/eprint/13277

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