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Angiogenic and inflammatory markers of cardiopulmonary changes in children and adolescents with sickle cell disease

Niu, X and Nouraie, M and Campbell, A and Rana, S and Minniti, CP and Sable, C and Darbari, D and Dham, N and Reading, NS and Prchal, JT and Kato, GJ and Gladwin, MT and Castro, OL and Gordeuk, VR (2009) Angiogenic and inflammatory markers of cardiopulmonary changes in children and adolescents with sickle cell disease. PLoS ONE, 4 (11).

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Background: Pulmonary hypertension and left ventricular diastolic dysfunction are complications of sickle cell disease. Pulmonary hypertension is associated with hemolysis and hypoxia, but other unidentified factors are likely involved in pathogenesis as well. Design and Methods: Plasma concentrations of three angiogenic markers (fibroblast growth factor, platelet derived growth factor-BB [PDGF-BB], vascular endothelial growth factor [VEGF]) and seven inflammatory markers implicated in pulmonary hypertension in other settings were determined by Bio-Plex suspension array in 237 children and adolescents with sickle cell disease at steady state and 43 controls. Tricuspid regurgitation velocity (which reflects systolic pulmonary artery pressure), mitral valve E/Edti ratio (which reflects left ventricular diastolic dysfunction), and a hemolytic component derived from four markers of hemolysis and hemoglobin oxygen saturation were also determined. Results: Plasma concentrations of interleukin-8, interleukin-10 and VEGF were elevated in the patients with sickle cell disease compared to controls (P≤0.003). By logistic regression, greater values for PDGF-BB (P = 0.009), interleukin-6 (P = 0.019) and the hemolytic component (P = 0.026) were independently associated with increased odds of elevated tricuspid regurgitation velocity while higher VEGF concentrations were associated with decreased odds (P = 0.005) among the patients with sickle cell disease. These findings, which are consistent with reports that PDGF-BB stimulates and VEGF inhibits vascular smooth muscle cell proliferation, did not apply to E/Etdi. Conclusions: Circulating concentrations of angiogenic and pro-Inflammatory markers are altered in sickle cell disease children and adolescents with elevated tricuspid regurgitation velocity, a subgroup that may be at risk for developing worsening pulmonary hypertension. Further studies to understand the molecular changes in these children are indicated.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Niu, X
Nouraie, M
Campbell, A
Rana, S
Minniti, CP
Sable, C
Darbari, D
Dham, N
Reading, NS
Prchal, JT
Kato, GJgjk22@pitt.eduGJK22
Gladwin, MTmtg16@pitt.eduMTG16
Castro, OL
Gordeuk, VR
ContributionContributors NameEmailPitt UsernameORCID
Date: 23 November 2009
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 4
Number: 11
DOI or Unique Handle: 10.1371/journal.pone.0007956
Refereed: Yes
MeSH Headings: Adolescent; Adult; Anemia, Sickle Cell--complications; Anemia, Sickle Cell--immunology; Anoxia; Cardiovascular Diseases--immunology; Child; Child, Preschool; Female; Hemoglobins--metabolism; Hemolysis; Humans; Inflammation; Interleukin-10--blood; Interleukin-8--blood; Male; Neovascularization, Pathologic; Vascular Endothelial Growth Factor A--blood; Vascular Endothelial Growth Factor A--metabolism
Other ID: NLM PMC2776981
PubMed Central ID: PMC2776981
PubMed ID: 19956689
Date Deposited: 03 Aug 2012 18:35
Last Modified: 31 Jul 2020 16:00


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