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CD28 down-regulation on circulating CD4 T-cells is associated with poor prognoses of patients with idiopathic pulmonary fibrosis

Gilani, SR and Vuga, LJ and Lindell, KO and Gibson, KF and Xue, J and Kaminski, N and Valentine, VG and Lindsay, EK and George, MP and Steele, C and Duncan, SR (2010) CD28 down-regulation on circulating CD4 T-cells is associated with poor prognoses of patients with idiopathic pulmonary fibrosis. PLoS ONE, 5 (1).

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Abstract

Background: Although the etiology of idiopathic pulmonary fibrosis (IPF) remains perplexing, adaptive immune activation is evident among many afflicted patients. Repeated cycles of antigen-induced proliferation cause T-cells to lose surface expression of CD28, and we hypothesized this process might also occur in IPF. Methodology/Principal Findings: Peripheral blood CD4 T-cells from 89 IPF patients were analyzed by flow cytometry and cytokine multiplex assays, and correlated with clinical events. In comparison to autologous CD4 +CD28+cells, the unusual CD4+CD28 null lymphocytes seen in many IPF patients had discordant expressions of activation markers, more frequently produced cytotoxic mediators perforin (2.4±0.8% vs. 60.0±7.4%, p<0.0001) and granzyme B (4.5±2.8% vs.74.9±6.5%, p<0.0001), produced greater amounts of many pro-inflammatory cytokines, and less frequently expressed the regulatory T-cell marker FoxP3 (12.9±1.1% vs. 3.3±0.6% p<0.0001). Infiltration of CD4+CD28null T-cells in IPF lungs was confirmed by confocal microscopy. Interval changes of CD28 expression among subjects who had replicate studies were correlated with conterminous changes of their forced vital capacities (rs = 0.49, p = 0.012). Most importantly, one-year freedom from major adverse clinical events (either death or lung transplantation) was 56±6% among 78 IPF patients with CD4 +CD28+/CD4total≥82%, compared to 9±9% among those with more extensive CD28 down-regulation (CD4+CD28 +/CD4total<82%) (p = 0.0004). The odds ratio for major adverse events among those with the most extensive CD28 down-regulation was 13.0, with 95% confidence intervals 1.6-111.1. Conclusions/Significance: Marked down-regulation of CD28 on circulating CD4 T-cells, a result of repeated antigen-driven proliferations, is associated with poor outcomes in IPF patients. The CD4+CD28null cells of these patients have potentially enhanced pathogenic characteristics, including increased productions of cytotoxic mediators and pro-inflammatory cytokines. These findings show proliferative T-cell responses to antigen(s) resulting in CD28 down-regulation are associated with progression and manifestations of IPF, and suggest assays of circulating CD4 T-cells may identify patients at greatest risk for clinical deterioration. © 2010 Gilani et al.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Gilani, SR
Vuga, LJlgv4@pitt.eduLGV4
Lindell, KOkol2@pitt.eduKOL2
Gibson, KFkfg@pitt.eduKFG
Xue, Jjix28@pitt.eduJIX28
Kaminski, N
Valentine, VG
Lindsay, EK
George, MP
Steele, C
Duncan, SR
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorGregson, AricUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 29 January 2010
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 5
Number: 1
DOI or Unique Handle: 10.1371/journal.pone.0008959
Refereed: Yes
MeSH Headings: Adult; Antigens, CD28--immunology; CD4-Positive T-Lymphocytes--immunology; Down-Regulation; Humans; Male; Prognosis; Pulmonary Fibrosis--immunology
Other ID: NLM PMC2813297
PubMed Central ID: PMC2813297
PubMed ID: 20126467
Date Deposited: 31 Jul 2012 20:47
Last Modified: 02 Feb 2019 16:58
URI: http://d-scholarship.pitt.edu/id/eprint/13314

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