Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

Study of muscle cell dedifferentiation after skeletal muscle injury of mice with a Cre-Lox system

Mu, X and Peng, H and Pan, H and Huard, J and Li, Y (2011) Study of muscle cell dedifferentiation after skeletal muscle injury of mice with a Cre-Lox system. PLoS ONE, 6 (2).

Published Version
Available under License : See the attached license file.

Download (889kB) | Preview
[img] Plain Text (licence)
Available under License : See the attached license file.

Download (1kB)


Background: Dedifferentiation of muscle cells in the tissue of mammals has yet to be observed. One of the challenges facing the study of skeletal muscle cell dedifferentiation is the availability of a reliable model that can confidentially distinguish differentiated cell populations of myotubes and non-fused mononuclear cells, including stem cells that can coexist within the population of cells being studied. Methodology/Principal Findings: In the current study, we created a Cre/Lox-β-galactosidase system, which can specifically tag differentiated multinuclear myotubes and myotube-generated mononuclear cells based on the activation of the marker gene, β-galactosidase. By using this system in an adult mouse model, we found that β-galactosidase positive mononuclear cells were generated from β-galactosidase positive multinuclear myofibers upon muscle injury. We also demonstrated that these mononuclear cells can develop into a variety of different muscle cell lineages, i.e., myoblasts, satellite cells, and muscle derived stem cells. Conclusions/Significance: These novel findings demonstrated, for the first time, that cellular dedifferentiation of skeletal muscle cells actually occurs in mammalian skeletal muscle following traumatic injury in vivo. © 2011 Mu et al.


Social Networking:
Share |


Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Mu, X
Peng, H
Pan, H
Huard, J
Li, Y
Date: 14 February 2011
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 6
Number: 2
DOI or Unique Handle: 10.1371/journal.pone.0016699
Schools and Programs: Swanson School of Engineering > Bioengineering
Refereed: Yes
MeSH Headings: Animals; Cell Dedifferentiation--genetics; Cell Dedifferentiation--physiology; Cell Tracking--methods; Integrases--genetics; Integrases--metabolism; Male; Mice; Mice, Inbred C57BL; Mice, SCID; Mice, Transgenic; Muscle Fibers, Skeletal--metabolism; Muscle Fibers, Skeletal--pathology; Muscle Fibers, Skeletal--physiology; Muscle, Skeletal--injuries; Muscle, Skeletal--metabolism; Muscle, Skeletal--pathology; Organ Specificity--genetics; Regeneration--physiology; Transgenes--physiology; beta-Galactosidase--genetics; beta-Galactosidase--metabolism
Other ID: NLM PMC3033395
PubMed Central ID: PMC3033395
PubMed ID: 21304901
Date Deposited: 03 Aug 2012 19:00
Last Modified: 13 Oct 2017 22:56


Monthly Views for the past 3 years

Plum Analytics

Actions (login required)

View Item View Item