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Organization of the cpe locus in CPE-positive Clostridium perfringens type C and D isolates

Li, J and Miyamoto, K and Sayeed, S and McClane, BA (2010) Organization of the cpe locus in CPE-positive Clostridium perfringens type C and D isolates. PLoS ONE, 5 (6).

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Clostridium perfringens enterotoxin (encoded by the cpe gene) contributes to several important human, and possibly veterinary, enteric diseases. The current study investigated whether cpe locus organization in type C or D isolates resembles one of the three (one chromosomal and two plasmid-borne) cpe loci commonly found amongst type A isolates. Multiplex PCR assays capable of detecting sequences in those type A cpe loci failed to amplify products from cpe-positive type C and D isolates, indicating these isolates possess different cpe locus arrangements. Therefore, restriction fragments containing the cpe gene were cloned and sequenced from two type C isolates and one type D isolate. The obtained cpe locus sequences were then used to construct an overlapping PCR assay to assess cpe locus diversity amongst other cpe-positive type C and D isolates. All seven surveyed cpe-positive type C isolates had a plasmid-borne cpe locus partially resembling the cpe locus of type A isolates carrying a chromosomal cpe gene. In contrast, all eight type D isolates shared the same plasmidborne cpe locus, which differed substantially from the cpe locus present in other C. perfringens by containing two copies of an ORF with 67% identity to a transposase gene (COG4644) found in Tn1546, but not previously associated with the cpe gene. These results identify greater diversity amongst cpe locus organization than previously appreciated, providing new insights into cpe locus evolution. Finally, evidence for cpe gene mobilization was found for both type C and D isolates, which could explain their cpe plasmid diversity. © 2010 Li et al.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Li, Jjihongli@pitt.eduJIHONGLI
Miyamoto, K
Sayeed, Ssas69@pitt.eduSAS69
McClane, BAbamcc@pitt.eduBAMCC
ContributionContributors NameEmailPitt UsernameORCID
Date: 9 August 2010
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 5
Number: 6
DOI or Unique Handle: 10.1371/journal.pone.0010932
Refereed: Yes
MeSH Headings: Base Sequence; Blotting, Southern; Clostridium perfringens--classification; Clostridium perfringens--genetics; Clostridium perfringens--isolation & purification; DNA Primers; Electrophoresis, Gel, Pulsed-Field; Enterotoxins; Genes, Bacterial; Open Reading Frames; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length
Other ID: NLM PMC2880595
PubMed Central ID: PMC2880595
PubMed ID: 20532170
Date Deposited: 03 Aug 2012 20:52
Last Modified: 02 Feb 2019 16:58


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