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Release of intracellular calcium stores facilitates coxsackievirus entry into polarized endothelial cells

Bozym, RA and Morosky, SA and Kim, KS and Cherry, S and Coyne, CB (2010) Release of intracellular calcium stores facilitates coxsackievirus entry into polarized endothelial cells. PLoS Pathogens, 6 (10). ISSN 1553-7366

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Group B coxsackieviruses (CVB) are associated with viral-induced heart disease and are among the leading causes of aseptic meningitis worldwide. Here we show that CVB entry into polarized brain microvasculature and aortic endothelial cells triggers a depletion of intracellular calcium stores initiated through viral attachment to the apical attachment factor decayaccelerating factor. Calcium release was dependent upon a signaling cascade that required the activity of the Src family of tyrosine kinases, phospholipase C, and the inositol 1,4,5-trisphosphate receptor isoform 3. CVB-mediated calcium release was required for the activation of calpain-2, a calcium-dependent cysteine protease, which controlled the vesicular trafficking of internalized CVB particles. These data point to a specific role for calcium signaling in CVB entry into polarized endothelial monolayers and highlight the unique signaling mechanisms used by these viruses to cross endothelial barriers. © 2010 Bozym et al.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Bozym, RA
Morosky, SAstm39@pitt.eduSTM39
Kim, KS
Cherry, S
Coyne, CB
ContributionContributors NameEmailPitt UsernameORCID
Date: 1 October 2010
Date Type: Publication
Journal or Publication Title: PLoS Pathogens
Volume: 6
Number: 10
DOI or Unique Handle: 10.1371/journal.ppat.1001135
Refereed: Yes
ISSN: 1553-7366
MeSH Headings: Calcium--metabolism; Calcium Signaling--physiology; Calpain--metabolism; Calpain--physiology; Cell Polarity--physiology; Cells, Cultured; Coxsackievirus Infections--metabolism; Endothelial Cells--metabolism; Endothelial Cells--physiology; Endothelial Cells--virology; Endothelium, Vascular--metabolism; Endothelium, Vascular--physiology; Endothelium, Vascular--virology; Enterovirus--physiology; Epithelium--metabolism; Epithelium--physiology; Epithelium--virology; Humans; Inositol 1,4,5-Trisphosphate Receptors--metabolism; Inositol 1,4,5-Trisphosphate Receptors--physiology; Intracellular Space--metabolism; Phospholipase C gamma--metabolism; Phospholipase C gamma--physiology; Virus Internalization
Other ID: NLM PMC2951373
PubMed Central ID: PMC2951373
PubMed ID: 20949071
Date Deposited: 22 Aug 2012 21:50
Last Modified: 23 Jan 2019 01:55


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