Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

Release of intracellular calcium stores facilitates coxsackievirus entry into polarized endothelial cells

Bozym, RA and Morosky, SA and Kim, KS and Cherry, S and Coyne, CB (2010) Release of intracellular calcium stores facilitates coxsackievirus entry into polarized endothelial cells. PLoS Pathogens, 6 (10). ISSN 1553-7366

[img]
Preview
PDF
Published Version
Available under License : See the attached license file.

Download (16MB) | Preview
[img] Plain Text (licence)
Available under License : See the attached license file.

Download (1kB)

Abstract

Group B coxsackieviruses (CVB) are associated with viral-induced heart disease and are among the leading causes of aseptic meningitis worldwide. Here we show that CVB entry into polarized brain microvasculature and aortic endothelial cells triggers a depletion of intracellular calcium stores initiated through viral attachment to the apical attachment factor decayaccelerating factor. Calcium release was dependent upon a signaling cascade that required the activity of the Src family of tyrosine kinases, phospholipase C, and the inositol 1,4,5-trisphosphate receptor isoform 3. CVB-mediated calcium release was required for the activation of calpain-2, a calcium-dependent cysteine protease, which controlled the vesicular trafficking of internalized CVB particles. These data point to a specific role for calcium signaling in CVB entry into polarized endothelial monolayers and highlight the unique signaling mechanisms used by these viruses to cross endothelial barriers. © 2010 Bozym et al.


Share

Citation/Export:
Social Networking:
Share |

Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Bozym, RA
Morosky, SAstm39@pitt.eduSTM39
Kim, KS
Cherry, S
Coyne, CB
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorHope, Thomas J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 1 October 2010
Date Type: Publication
Journal or Publication Title: PLoS Pathogens
Volume: 6
Number: 10
DOI or Unique Handle: 10.1371/journal.ppat.1001135
Refereed: Yes
ISSN: 1553-7366
MeSH Headings: Calcium--metabolism; Calcium Signaling--physiology; Calpain--metabolism; Calpain--physiology; Cell Polarity--physiology; Cells, Cultured; Coxsackievirus Infections--metabolism; Endothelial Cells--metabolism; Endothelial Cells--physiology; Endothelial Cells--virology; Endothelium, Vascular--metabolism; Endothelium, Vascular--physiology; Endothelium, Vascular--virology; Enterovirus--physiology; Epithelium--metabolism; Epithelium--physiology; Epithelium--virology; Humans; Inositol 1,4,5-Trisphosphate Receptors--metabolism; Inositol 1,4,5-Trisphosphate Receptors--physiology; Intracellular Space--metabolism; Phospholipase C gamma--metabolism; Phospholipase C gamma--physiology; Virus Internalization
Other ID: NLM PMC2951373
PubMed Central ID: PMC2951373
PubMed ID: 20949071
Date Deposited: 22 Aug 2012 21:50
Last Modified: 23 Jan 2019 01:55
URI: http://d-scholarship.pitt.edu/id/eprint/13556

Metrics

Monthly Views for the past 3 years

Plum Analytics

Altmetric.com


Actions (login required)

View Item View Item