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Gonadal steroid modulation of oxytocin and vasopressin gene expression in the hypothalamus of the osmotically stimulated rat.

Crowley, Rebecca S and Amico, JA (1993) Gonadal steroid modulation of oxytocin and vasopressin gene expression in the hypothalamus of the osmotically stimulated rat. Endocrinology, 133 (6). 2711 - 2718. ISSN 0013-7227

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Abstract

We investigated the modulatory role of gonadal steroids on the expression of oxytocin (OT) and vasopressin (AVP) cytoplasmic mRNAs in the paraventricular nucleus and supraoptic nucleus of the osmotically stimulated rat. We chronically administered an oral salt load (2% sodium chloride solution for 5 days) to intact and gonadectomized female and male Sprague-Dawley rats and measured serum sodium, body weight, pituitary content of OT and AVP immunoreactivities, and size and abundance of hypothalamic cytoplasmic OT and AVP mRNA transcripts. Intact and gonadectomized rats that were administered an osmotic challenge developed comparable degrees of hypernatremia and loss of body weight as well as depletion of posterior pituitary stores of OT and AVP. Hyperosmolality induced elongation of the OT and AVP transcripts in intact and gonadectomized animals, but only intact rats had enhanced hypothalamic cytoplasmic OT and AVP mRNA concentrations to this stimulus. Replacement with gonadal steroids restored the up-regulation in OT and AVP gene expression in gonadectomized animals rendered hyperosmolar. The findings support a modulatory role for gonadal steroids in hypothalamic OT and AVP gene expression during osmotic stimulation.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Crowley, Rebecca S
Amico, JA
Date: December 1993
Date Type: Publication
Journal or Publication Title: Endocrinology
Volume: 133
Number: 6
Page Range: 2711 - 2718
Schools and Programs: School of Medicine > Biomedical Informatics
Refereed: Yes
ISSN: 0013-7227
Article Type: Research Article
MeSH Headings: Animals; Arginine Vasopressin--genetics; Castration; Estradiol--blood; Female; Gene Expression--physiology; Gonadal Steroid Hormones--physiology; Hypothalamus--physiology; Male; Osmosis; Oxytocin--genetics; Progesterone--blood; RNA, Messenger--metabolism; Rats; Rats, Sprague-Dawley; Saline Solution, Hypertonic--pharmacology; Testosterone--pharmacology
PubMed ID: 8243294
Date Deposited: 29 Aug 2012 20:19
Last Modified: 01 Nov 2017 14:03
URI: http://d-scholarship.pitt.edu/id/eprint/13780

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