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Increased accumulation of oxytocin messenger ribonucleic acid in the hypothalamus of the female rat: induction by long term estradiol and progesterone administration and subsequent progesterone withdrawal.

Crowley, RS and Insel, TR and O'Keefe, JA and Kim, NB and Amico, JA (1995) Increased accumulation of oxytocin messenger ribonucleic acid in the hypothalamus of the female rat: induction by long term estradiol and progesterone administration and subsequent progesterone withdrawal. Endocrinology, 136 (1). 224 - 231. ISSN 0013-7227

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Abstract

To examine a possible role for gonadal steroid hormones in the enhanced accumulation of hypothalamic oxytocin (OT) messenger RNA (mRNA) and peptide in late pregnancy, we used an established model (22) in which sequential administration of estradiol (E2) and progesterone (P) SILASTIC capsules to ovariectomized rats is followed by removal of P. Long term and sustained E2 combined with abrupt P withdrawal mimics the gonadal steroid hormone pattern of late gestation in the rat (22). Using this paradigm, we demonstrate that OT mRNA is increased in the rat hypothalamus after long term P treatment, but only in the presence of E2 and only when P capsules are removed 48 h before killing. Furthermore, we show that P replacement in primiparous rats during late pregnancy blunts the increase in OT mRNA normally observed at the end of gestation. Our results support a role for E2 priming and P withdrawal in the enhanced accumulation of OT mRNA in the hypothalamus of the female rat.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Crowley, RS
Insel, TR
O'Keefe, JA
Kim, NB
Amico, JA
Date: January 1995
Date Type: Publication
Journal or Publication Title: Endocrinology
Volume: 136
Number: 1
Page Range: 224 - 231
DOI or Unique Handle: 10.1210/endo.136.1.7828535
Schools and Programs: School of Medicine > Biomedical Informatics
Refereed: Yes
Uncontrolled Keywords: Animals, Arginine Vasopressin, Drug Implants, Estradiol, Female, Hypothalamus, Ovariectomy, Oxytocin, Pituitary Gland, Pregnancy, Progesterone, RNA, Messenger, Rats, Sex Characteristics, Substance Withdrawal Syndrome, Time Factors
ISSN: 0013-7227
Funders: NIDDK NIH HHS (FT32-DK-0752)
MeSH Headings: Animals; Arginine Vasopressin--genetics; Arginine Vasopressin--metabolism; Drug Implants; Estradiol--blood; Estradiol--pharmacology; Female; Hypothalamus--metabolism; Ovariectomy; Oxytocin--genetics; Oxytocin--metabolism; Pituitary Gland--metabolism; Pregnancy; Progesterone--blood; Progesterone--pharmacology; RNA, Messenger--metabolism; Rats; Sex Characteristics; Substance Withdrawal Syndrome--metabolism; Time Factors
PubMed ID: 7828535
Date Deposited: 29 Aug 2012 20:16
Last Modified: 14 Oct 2017 05:57
URI: http://d-scholarship.pitt.edu/id/eprint/13782

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