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Bacterial chemotaxis in an optical trap

Altindal, T and Chattopadhyay, S and Wu, XL (2011) Bacterial chemotaxis in an optical trap. PLoS ONE, 6 (4).

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Abstract

An optical trapping technique is implemented to investigate the chemotactic behavior of a marine bacterial strain Vibrio alginolyticus. The technique takes the advantage that the bacterium has only a single polar flagellum, which can rotate either in the counter-clock-wise or clock-wise direction. The two rotation states of the motor can be readily and instantaneously resolved in the optical trap, allowing the flagellar motor switching rate S(t)to be measured under different chemical stimulations. In this paper the focus will be on the bacterial response to an impulsive change of chemoattractant serine. Despite different propulsion apparati and motility patterns, cells of V. alginolyticus apparently use a similar response as Escherichia coli to regulate their chemotactic behavior. Specifically, we found that the switching rate S(t) of the bacterial motor exhibits a biphasic behavior, showing a fast initial response followed by a slow relaxation to the steady-state switching rate S0. The measured S(t)can be mimicked by a model that has been recently proposed for chemotaxis in E. coli. The similarity in the response to the brief chemical stimulation in these two different bacteria is striking, suggesting that the biphasic response may be evolutionarily conserved. This study also demonstrated that optical tweezers can be a useful tool for chemotaxis studies and should be applicable to other polarly flagellated bacteria. © 2011 Altindal et al.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Altindal, T
Chattopadhyay, S
Wu, XLxlwu@pitt.eduXLWU
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorHansen, Immo A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 20 April 2011
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 6
Number: 4
DOI or Unique Handle: 10.1371/journal.pone.0018231
Schools and Programs: Dietrich School of Arts and Sciences > Astronomy
Dietrich School of Arts and Sciences > Physics
Refereed: Yes
MeSH Headings: Chemotactic Factors--pharmacology; Chemotaxis--drug effects; Flagella--drug effects; Flagella--metabolism; Models, Biological; Optical Tweezers; Rotation; Serine--pharmacology; Time Factors; Vibrio alginolyticus--cytology; Vibrio alginolyticus--drug effects
Other ID: NLM PMC3072974
PubMed Central ID: PMC3072974
PubMed ID: 21494608
Date Deposited: 29 Aug 2012 21:14
Last Modified: 19 Jun 2021 10:55
URI: http://d-scholarship.pitt.edu/id/eprint/13820

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