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Lhx1 is required for specification of the renal progenitor cell field

Cirio, MC and Hui, Z and Haldin, CE and Cosentino, CC and Stuckenholz, C and Chen, X and Hong, SK and Dawid, IB and Hukriede, NA (2011) Lhx1 is required for specification of the renal progenitor cell field. PLoS ONE, 6 (4).

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Abstract

In the vertebrate embryo, the kidney is derived from the intermediate mesoderm. The LIM-class homeobox transcription factor lhx1 is expressed early in the intermediate mesoderm and is one of the first genes to be expressed in the nephric mesenchyme. In this study, we investigated the role of Lhx1 in specification of the kidney field by either overexpressing or depleting lhx1 in Xenopus embryos or depleting lhx1 in an explant culture system. By overexpressing a constitutively-active form of Lhx1, we established its capacity to expand the kidney field during the specification stage of kidney organogenesis. In addition, the ability of Lhx1 to expand the kidney field diminishes as kidney organogenesis transitions to the morphogenesis stage. In a complimentary set of experiments, we determined that embryos depleted of lhx1, show an almost complete loss of the kidney field. Using an explant culture system to induce kidney tissue, we confirmed that expression of genes from both proximal and distal kidney structures is affected by the absence of lhx1. Taken together our results demonstrate an essential role for Lhx1 in driving specification of the entire kidney field from the intermediate mesoderm. © 2011.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Cirio, MCmcc19@pitt.eduMCC19
Hui, Z
Haldin, CE
Cosentino, CC
Stuckenholz, Ccstucken@pitt.eduCSTUCKEN
Chen, X
Hong, SK
Dawid, IB
Hukriede, NAhukriede@pitt.eduHUKRIEDE
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorRoberts, Roland G.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 25 April 2011
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 6
Number: 4
DOI or Unique Handle: 10.1371/journal.pone.0018858
Schools and Programs: School of Medicine > Developmental Biology
Refereed: Yes
MeSH Headings: Animals; Body Patterning--genetics; Cell Proliferation; Embryo, Nonmammalian--metabolism; Gene Expression Regulation, Developmental; Gene Silencing; Homeodomain Proteins--genetics; Homeodomain Proteins--metabolism; Kidney--cytology; LIM-Homeodomain Proteins; Mesoderm--cytology; Oligonucleotide Array Sequence Analysis; Oligonucleotides, Antisense--pharmacology; Organ Culture Techniques; Stem Cells--cytology; Stem Cells--metabolism; Time Factors; Transcription Factors; Xenopus--embryology; Xenopus--genetics; Xenopus Proteins--deficiency; Xenopus Proteins--genetics; Xenopus Proteins--metabolism
Other ID: NLM PMC3078140
PubMed Central ID: PMC3078140
PubMed ID: 21526205
Date Deposited: 29 Aug 2012 21:15
Last Modified: 05 Feb 2019 16:55
URI: http://d-scholarship.pitt.edu/id/eprint/13826

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