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Identification of novel clostridium perfringens type E strains that carry an iota toxin plasmid with a functional enterotoxin gene

Miyamoto, K and Yumine, N and Mimura, K and Nagahama, M and Li, J and McClane, BA and Akimoto, S (2011) Identification of novel clostridium perfringens type E strains that carry an iota toxin plasmid with a functional enterotoxin gene. PLoS ONE, 6 (5).

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Abstract

Clostridium perfringens enterotoxin (CPE) is a major virulence factor for human gastrointestinal diseases, such as food poisoning and antibiotic associated diarrhea. The CPE-encoding gene (cpe) can be chromosomal or plasmid-borne. Recent development of conventional PCR cpe-genotyping assays makes it possible to identify cpe location (chromosomal or plasmid) in type A isolates. Initial studies for developing cpe genotyping assays indicated that all cpe-positive strains isolated from sickened patients were typable by cpe-genotypes, but surveys of C. perfringens environmental strains or strains from feces of healthy people suggested that this assay might not be useful for some cpe-carrying type A isolates. In the current study, a pulsed-field gel electrophoresis Southern blot assay showed that four cpe-genotype untypable isolates carried their cpe gene on a plasmid of ~65 kb. Complete sequence analysis of the ~65 kb variant cpe-carrying plasmid revealed no intact IS elements and a disrupted cytosine methyltransferase (dcm) gene. More importantly, this plasmid contains a conjugative transfer region, a variant cpe gene and variant iota toxin genes. The toxin genes encoded by this plasmid are expressed based upon the results of RT-PCR assays. The ~65 kb plasmid is closely related to the pCPF4969 cpe plasmid of type A isolates. MLST analyses indicated these isolates belong to a unique cluster of C. perfringens. Overall, these isolates carrying a variant functional cpe gene and iota toxin genes represent unique type E strains. © 2011 Miyamoto et al.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Miyamoto, K
Yumine, N
Mimura, K
Nagahama, M
Li, Jjihongli@pitt.eduJIHONGLI
McClane, BAbamcc@pitt.eduBAMCC
Akimoto, S
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorTse, HermanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 6 June 2011
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 6
Number: 5
DOI or Unique Handle: 10.1371/journal.pone.0020376
Schools and Programs: School of Medicine > Microbiology and Molecular Genetics
Refereed: Yes
MeSH Headings: ADP Ribose Transferases--genetics; Bacterial Toxins--genetics; Clostridium perfringens--genetics; Enterotoxins--genetics; Molecular Sequence Data; Plasmids--genetics; Reverse Transcriptase Polymerase Chain Reaction
Other ID: NLM PMC3105049
PubMed Central ID: PMC3105049
PubMed ID: 21655254
Date Deposited: 30 Aug 2012 15:00
Last Modified: 02 Feb 2019 16:58
URI: http://d-scholarship.pitt.edu/id/eprint/13862

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