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Venous graft-derived cells participate in peripheral nerve regeneration

Lavasani, M and Gehrmann, S and Gharaibeh, B and Clark, KA and Kaufmann, RA and Péault, B and Goitz, RJ and Huard, J (2011) Venous graft-derived cells participate in peripheral nerve regeneration. PLoS ONE, 6 (9).

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Abstract

Background: Based on growing evidence that some adult multipotent cells necessary for tissue regeneration reside in the walls of blood vessels and the clinical success of vein wrapping for functional repair of nerve damage, we hypothesized that the repair of nerves via vein wrapping is mediated by cells migrating from the implanted venous grafts into the nerve bundle. Methodology/Principal Findings: To test the hypothesis, severed femoral nerves of rats were grafted with venous grafts from animals of the opposite sex. Nerve regeneration was impaired when decellularized or irradiated venous grafts were used in comparison to untreated grafts, supporting the involvement of venous graft-derived cells in peripheral nerve repair. Donor cells bearing Y chromosomes integrated into the area of the host injured nerve and participated in remyelination and nerve regeneration. The regenerated nerve exhibited proper axonal myelination, and expressed neuronal and glial cell markers. Conclusions/Significance: These novel findings identify the mechanism by which vein wrapping promotes nerve regeneration. © 2011 Lavasani et al.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Lavasani, M
Gehrmann, S
Gharaibeh, Bburhan@pitt.eduBURHAN0000-0002-5947-1232
Clark, KAkac220@pitt.eduKAC220
Kaufmann, RArak30@pitt.eduRAK30
Péault, B
Goitz, RJrog2@pitt.eduROG2
Huard, J
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorChin, Wei-ChunUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Centers: Other Centers, Institutes, Offices, or Units > Center for Biologic Imaging
Date: 23 September 2011
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 6
Number: 9
DOI or Unique Handle: 10.1371/journal.pone.0024801
Schools and Programs: School of Medicine > Biochemistry and Molecular Genetics
School of Medicine > Cell Biology and Molecular Physiology
School of Medicine > Orthopaedic Surgery
School of Medicine > Pediatrics
Swanson School of Engineering > Bioengineering
Refereed: Yes
MeSH Headings: Animals; Axons--metabolism; Axons--ultrastructure; Cell Movement; Cell Proliferation; Cells, Cultured; Female; Femoral Nerve--injuries; Femoral Nerve--physiopathology; Femoral Nerve--surgery; Femoral Vein--cytology; Femoral Vein--radiation effects; Femoral Vein--transplantation; Immunohistochemistry; In Situ Hybridization, Fluorescence; Male; Microscopy, Electron, Transmission; Nerve Fibers, Myelinated--metabolism; Nerve Fibers, Myelinated--ultrastructure; Nerve Regeneration; Neurofilament Proteins--metabolism; Rats; Rats, Inbred F344; Schwann Cells--metabolism; Schwann Cells--ultrastructure; Sex Factors; Transplantation, Homologous
Other ID: NLM PMC3179479
PubMed Central ID: PMC3179479
PubMed ID: 21966370
Date Deposited: 04 Sep 2012 14:42
Last Modified: 04 Feb 2019 16:55
URI: http://d-scholarship.pitt.edu/id/eprint/13897

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