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Functional connection between Rad51 and PML in homology-directed repair

Boichuk, S and Hu, L and Makielski, K and Pandolfi, PP and Gjoerup, OV (2011) Functional connection between Rad51 and PML in homology-directed repair. PLoS ONE, 6 (10).

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The promyelocytic leukemia protein (PML) is a tumor suppressor critical for formation of nuclear bodies (NBs) performing important functions in transcription, apoptosis, DNA repair and antiviral responses. Earlier studies demonstrated that simian virus 40 (SV40) initiates replication near PML NBs. Here we show that PML knockdown inhibits viral replication in vivo, thus indicating a positive role of PML early in infection. SV40 large T antigen (LT) induces DNA damage and, consequently, nuclear foci of the key homologous recombination repair protein Rad51 that colocalize with PML. PML depletion abrogates LT-induced Rad51 foci. LT may target PML NBs to gain access to DNA repair factors like Rad51 that are required for viral replication. We have used the SV40 model to gain insight to DNA repair events involving PML. Strikingly, even in normal cells devoid of viral oncoproteins, PML is found to be instrumental for foci of Rad51, Mre11 and BRCA1, as well as homology-directed repair after double-strand break (DSB) induction. Following LT expression or external DNA damage, PML associates with Rad51. PML depletion also causes a loss of RPA foci following γ-irradiation, suggesting that PML is required for processing of DSBs. Immunofluorescent detection of incorporated BrdU without prior denaturation indicates a failure to generate ssDNA foci in PML knockdown cells upon γ-irradiation. Consistent with the lack of RPA and BrdU foci, γ-irradiation fails to induce Chk1 activation, when PML is depleted. Taken together, we have discovered a novel functional connection between PML and the homologous recombination-mediated repair machinery, which might contribute to PML tumor suppressor activity. © 2011 Boichuk et al.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Boichuk, S
Hu, L
Makielski, K
Pandolfi, PP
Gjoerup, OV
ContributionContributors NameEmailPitt UsernameORCID
Centers: Other Centers, Institutes, Offices, or Units > Pittsburgh Cancer Institute
Date: 5 October 2011
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 6
Number: 10
DOI or Unique Handle: 10.1371/journal.pone.0025814
Refereed: Yes
MeSH Headings: Animals; Antigens, Viral, Tumor--metabolism; BRCA1 Protein--metabolism; COS Cells; Cell Nucleus--metabolism; Cell Nucleus--virology; Cercopithecus aethiops; DNA Breaks, Double-Stranded; DNA-Binding Proteins--metabolism; Enzyme Activation; Gene Expression Regulation; Gene Knockdown Techniques; Humans; Mice; Nuclear Proteins--deficiency; Nuclear Proteins--genetics; Nuclear Proteins--metabolism; Protein Kinases--metabolism; Protein Stability; Protein Transport; Rad51 Recombinase--chemistry; Rad51 Recombinase--metabolism; Recombinational DNA Repair; Replication Protein A--metabolism; Simian virus 40--immunology; Simian virus 40--physiology; Transcription Factors--deficiency; Transcription Factors--genetics; Transcription Factors--metabolism; Tumor Suppressor Proteins--deficiency; Tumor Suppressor Proteins--genetics; Tumor Suppressor Proteins--metabolism; Virus Replication
Other ID: NLM PMC3187806
PubMed Central ID: PMC3187806
PubMed ID: 21998700
Date Deposited: 10 Sep 2012 14:35
Last Modified: 02 Feb 2019 14:55


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