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Population based model of human embryonic stem cell (hESC) differentiation during endoderm induction

Task, K and Jaramillo, M and Banerjee, I (2012) Population based model of human embryonic stem cell (hESC) differentiation during endoderm induction.

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The mechanisms by which human embryonic stem cells (hESC) differentiate to endodermal lineage have not been extensively studied. Mathematical models can aid in the identification of mechanistic information. In this work we use a population-based modeling approach to understand the mechanism of endoderm induction in hESC, performed experimentally with exposure to Activin A and Activin A supplemented with growth factors (basic fibroblast growth factor (FGF2) and bone morphogenetic protein 4 (BMP4)). The differentiating cell population is analyzed daily for cellular growth, cell death, and expression of the endoderm proteins Sox17 and CXCR4. The stochastic model starts with a population of undifferentiated cells, wherefrom it evolves in time by assigning each cell a propensity to proliferate, die and differentiate using certain user defined rules. Twelve alternate mechanisms which might describe the observed dynamics were simulated, and an ensemble parameter estimation was performed on each mechanism. A comparison of the quality of agreement of experimental data with simulations for several competing mechanisms led to the identification of one which adequately describes the observed dynamics under both induction conditions. The results indicate that hESC commitment to endoderm occurs through an intermediate mesendoderm germ layer which further differentiates into mesoderm and endoderm, and that during induction proliferation of the endoderm germ layer is promoted. Furthermore, our model suggests that CXCR4 is expressed in mesendoderm and endoderm, but is not expressed in mesoderm. Comparison between the two induction conditions indicates that supplementing FGF2 and BMP4 to Activin A enhances the kinetics of differentiation than Activin A alone. This mechanistic information can aid in the derivation of functional, mature cells from their progenitors. While applied to initial endoderm commitment of hESC, the model is general enough to be applicable either to a system of adult stem cells or later stages of ESC differentiation. © 2012 Task et al.


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Item Type: Article
CreatorsEmailPitt UsernameORCID
Task, K
Jaramillo, M
Banerjee, Iipb1@pitt.eduIPB1
ContributionContributors NameEmailPitt UsernameORCID
Date: 12 March 2012
Date Type: Publication
DOI or Unique Handle: 10.1371/journal.pone.0032975
Schools and Programs: Swanson School of Engineering > Bioengineering
Swanson School of Engineering > Chemical Engineering
Refereed: Yes
MeSH Headings: Activins--metabolism; Bone Morphogenetic Protein 4--metabolism; Cell Differentiation--physiology; Cell Line; DNA Primers--genetics; Embryonic Stem Cells--physiology; Endoderm--cytology; Endoderm--embryology; Fibroblast Growth Factor 2--metabolism; Flow Cytometry; Humans; Models, Biological; Receptors, CXCR4--metabolism; Reverse Transcriptase Polymerase Chain Reaction; SOXF Transcription Factors--metabolism
Other ID: NLM PMC3299713
PubMed Central ID: PMC3299713
PubMed ID: 22427920
Date Deposited: 13 Sep 2012 19:16
Last Modified: 02 Feb 2019 15:55


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