Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

Molecular epidemiology of neisseria meningitidis serogroup B in Brazil

de Filippis, I and de Lemos, APS and Hostetler, JB and Wollenberg, K and Sacchi, CT and Harrison, LH and Bash, MC and Prevots, DR (2012) Molecular epidemiology of neisseria meningitidis serogroup B in Brazil. PLoS ONE, 7 (3).

Published Version
Available under License : See the attached license file.

Download (1MB) | Preview
[img] Plain Text (licence)
Available under License : See the attached license file.

Download (1kB)


Background: Neisseria meningitidis serogroup B has been predominant in Brazil, but no broadly effective vaccine is available to prevent endemic meningococcal disease. To understand genetic diversity among serogroup B strains in Brazil, we selected a nationally representative sample of clinical disease isolates from 2004, and a temporally representative sample for the state of São Paulo (1988-2006) for study (n = 372). Methods: We performed multi-locus sequence typing (MLST) and sequence analysis of five outer membrane protein (OMP) genes, including novel vaccine targets fHbp and nadA. Results: In 2004, strain B:4:P1.15,19 clonal complex ST-32/ET-5 (cc32) predominated throughout Brazil; regional variation in MLST sequence type (ST), fetA, and porB was significant but diversity was limited for nadA and fHbp. Between 1988 and 1996, the São Paulo isolates shifted from clonal complex ST-41/44/Lineage 3 (cc41/44) to cc32. OMP variation was associated with but not predicted by cc or ST. Overall, fHbp variant 1/subfamily B was present in 80% of isolates and showed little diversity. The majority of nadA were similar to reference allele 1. Conclusions: A predominant serogroup B lineage has circulated in Brazil for over a decade with significant regional and temporal diversity in ST, fetA, and porB, but not in nadA and fHbp.


Social Networking:
Share |


Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
de Filippis, I
de Lemos, APS
Hostetler, JB
Wollenberg, K
Sacchi, CT
Harrison, LHlharriso@edc.pitt.eduLHARRISO
Bash, MC
Prevots, DR
ContributionContributors NameEmailPitt UsernameORCID
Date: 14 March 2012
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 7
Number: 3
DOI or Unique Handle: 10.1371/journal.pone.0033016
Schools and Programs: Graduate School of Public Health > Infectious Diseases and Microbiology
Refereed: Yes
MeSH Headings: Bacterial Typing Techniques; Base Sequence; Biodiversity; Brazil--epidemiology; Genes, Bacterial--genetics; Genetic Variation; Geography; Humans; Likelihood Functions; Meningococcal Infections--epidemiology; Meningococcal Infections--genetics; Meningococcal Infections--microbiology; Molecular Epidemiology; Molecular Sequence Data; Multilocus Sequence Typing; Neisseria meningitidis, Serogroup B--classification; Neisseria meningitidis, Serogroup B--genetics; Neisseria meningitidis, Serogroup B--isolation & purification; Phylogeny; Time Factors
Other ID: NLM PMC3303791
PubMed Central ID: PMC3303791
PubMed ID: 22431994
Date Deposited: 13 Sep 2012 20:24
Last Modified: 04 Feb 2019 15:56


Monthly Views for the past 3 years

Plum Analytics

Actions (login required)

View Item View Item