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Impaired clearance and enhanced pulmonary inflammatory/fibrotic response to carbon nanotubes in myeloperoxidase-deficient mice

Shvedova, AA and Kapralov, AA and Feng, WH and Kisin, ER and Murray, AR and Mercer, RR and St Croix, CM and Lang, MA and Watkins, SC and Konduru, NV and Allen, BL and Conroy, J and Kotchey, GP and Mohamed, BM and Meade, AD and Volkov, Y and Star, A and Fadeel, B and Kagan, VE (2012) Impaired clearance and enhanced pulmonary inflammatory/fibrotic response to carbon nanotubes in myeloperoxidase-deficient mice. PLoS ONE, 7 (3).

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Advancement of biomedical applications of carbonaceous nanomaterials is hampered by their biopersistence and pro-inflammatory action in vivo. Here, we used myeloperoxidase knockout B6.129X1-MPO (MPO k/o) mice and showed that oxidation and clearance of single walled carbon nanotubes (SWCNT) from the lungs of these animals after pharyngeal aspiration was markedly less effective whereas the inflammatory response was more robust than in wild-type C57Bl/6 mice. Our results provide direct evidence for the participation of MPO - one of the key-orchestrators of inflammatory response - in the in vivo pulmonary oxidative biodegradation of SWCNT and suggest new ways to control the biopersistence of nanomaterials through genetic or pharmacological manipulations. © 2012.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Shvedova, AA
Kapralov, AA
Feng, WH
Kisin, ER
Murray, AR
Mercer, RR
St Croix, CMclaudette.stcroix@pitt.eduCLS13
Lang, MA
Watkins, SCsimon.watkins@pitt.eduSWATKINS
Konduru, NV
Allen, BL
Conroy, J
Kotchey, GP
Mohamed, BM
Meade, AD
Volkov, Y
Star, Aastar@pitt.eduASTAR
Fadeel, B
Kagan, VEkagan@pitt.eduKAGAN
ContributionContributors NameEmailPitt UsernameORCID
Date: 30 March 2012
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 7
Number: 3
DOI or Unique Handle: 10.1371/journal.pone.0030923
Schools and Programs: Graduate School of Public Health > Environmental and Occupational Health
Dietrich School of Arts and Sciences > Chemistry
School of Medicine > Cell Biology and Molecular Physiology
Refereed: Yes
MeSH Headings: Animals; Bronchoalveolar Lavage Fluid--cytology; Chemokine CCL2--metabolism; Female; Fibrosis--chemically induced; Fibrosis--metabolism; Interleukin-6--metabolism; Lung--drug effects; Lung--metabolism; Lung--pathology; Mice; Mice, 129 Strain; Mice, Inbred C57BL; Mice, Knockout; Microscopy, Electron, Transmission; Microscopy, Fluorescence; Nanotubes, Carbon--toxicity; Nanotubes, Carbon--ultrastructure; Neutrophils--drug effects; Neutrophils--metabolism; Oxidation-Reduction; Peroxidase--deficiency; Peroxidase--genetics; Pneumonia--chemically induced; Pneumonia--metabolism; Spectrum Analysis, Raman; Tumor Necrosis Factor-alpha--metabolism
Other ID: NLM PMC3316527
PubMed Central ID: PMC3316527
PubMed ID: 22479306
Date Deposited: 24 Sep 2012 20:04
Last Modified: 30 Oct 2018 14:01


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