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Interferon regulatory factor 6 (IRF6) gene variants and the risk of isolated cleft lip or palate

Zucchero, TM and Cooper, ME and Maher, BS and Daack-Hirsch, S and Nepomuceno, B and Ribeiro, L and Caprau, D and Christensen, K and Suzuki, Y and Machida, J and Natsume, N and Yoshiura, KI and Vieira, AR and Orioli, IM and Castilla, EE and Moreno, L and Arcos-Burgos, M and Lidral, AC and Field, LL and Liu, YE and Ray, A and Goldstein, TH and Schultz, RE and Shi, M and Johnson, MK and Kondo, S and Schutte, BC and Marazita, ML and Murray, JC (2004) Interferon regulatory factor 6 (IRF6) gene variants and the risk of isolated cleft lip or palate. New England Journal of Medicine, 351 (8). 769 - 780. ISSN 0028-4793

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BACKGROUND: Cleft lip or palate (or the two in combination) is a common birth defect that results from a mixture of genetic and environmental factors. We searched for a specific genetic factor contributing to this complex trait by examining large numbers of affected patients and families and evaluating a specific candidate gene. METHODS: We identified the gene that encodes interferon regulatory factor 6 (IRF6) as a candidate gene on the basis of its involvement in an autosomal dominant form of cleft lip and palate, Van der Woude's syndrome. A single-nucleotide polymorphism in this gene results in either a valine or an isoleucine at amino acid position 274 (V274I). We carried out transmission-disequilibrium testing for V274I in 8003 individual subjects in 1968 families derived from 10 populations with ancestry in Asia, Europe, and South America, haplotype and linkage analyses, and case-control analyses, and determined the risk of cleft lip or palate that is associated with genetic variation in IRF6. RESULTS: Strong evidence of overtransmission of the valine (V) allele was found in the entire population data set (P<10-9); moreover, the results for some individual populations from South America and Asia were highly significant. Variation at IRF6 was responsible for 12 percent of the genetic contribution to cleft lip or palate and tripled the risk of recurrence in families that had already had one affected child. CONCLUSIONS: DNA-sequence variants associated with IRF6 are major contributors to cleft lip, with or without cleft palate. The contribution of variants in single genes to cleft lip or palate is an important consideration in genetic counseling. Copyright © 2004 Massachusetts Medical Society.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Zucchero, TM
Cooper, ME
Maher, BS
Daack-Hirsch, S
Nepomuceno, B
Ribeiro, L
Caprau, D
Christensen, K
Suzuki, Y
Machida, J
Natsume, N
Yoshiura, KI
Vieira, ARarv11@pitt.eduARV11
Orioli, IM
Castilla, EE
Moreno, L
Arcos-Burgos, M
Lidral, AC
Field, LL
Liu, YE
Ray, A
Goldstein, TH
Schultz, RE
Shi, M
Johnson, MK
Kondo, S
Schutte, BC
Marazita, MLmarazita@pitt.eduMARAZITA
Murray, JC
Date: 19 August 2004
Date Type: Publication
Journal or Publication Title: New England Journal of Medicine
Volume: 351
Number: 8
Page Range: 769 - 780
DOI or Unique Handle: 10.1056/nejmoa032909
Schools and Programs: School of Dental Medicine > Dental Science
Refereed: Yes
ISSN: 0028-4793
MeSH Headings: Cleft Lip--genetics; Cleft Palate--genetics; Continental Population Groups; DNA-Binding Proteins--genetics; Genotype; Haplotypes; Humans; Interferon Regulatory Factors; Linkage Disequilibrium; Pedigree; Polymorphism, Genetic; Risk Factors; Transcription Factors--genetics; Valine
PubMed ID: 15317890
Date Deposited: 18 Sep 2012 14:49
Last Modified: 22 Jun 2021 14:56


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