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Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood.

Vieira, Alexandre R and Deeley, Kathleen B and Callahan, Nicholas F and Noel, Jacqueline B and Anjomshoaa, Ida and Carricato, Wendy M and Schulhof, Louise P and Desensi, Rebecca S and Gandhi, Pooja and Resick, Judith M and Brandon, Carla A and Rozhon, Christopher and Patir, Asli and Yildirim, Mine and Poletta, Fernando A and Mereb, Juan C and Letra, Ariadne and Menezes, Renato and Wendell, Steven and Lopez-Camelo, Jorge S and Castilla, Eduardo E and Orioli, Iêda M and Seymen, Figen and Weyant, Robert J and Crout, Richard and McNeil, Daniel W and Modesto, Adriana and Marazita, Mary L (2011) Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood. ISRN Dent, 2011. 543561 - ?.

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Abstract

Caries is a multifactorial disease, and studies aiming to unravel the factors modulating its etiology must consider all known predisposing factors. One major factor is bacterial colonization, and Streptococcus mutans is the main microorganism associated with the initiation of the disease. In our studies, we have access to DNA samples extracted from human saliva and blood. In this report, we tested a real-time PCR assay developed to detect copies of genomic DNA from Streptococcus mutans in 1,424 DNA samples from humans. Our results suggest that we can determine the presence of genomic DNA copies of Streptococcus mutans in both DNA samples from caries-free and caries-affected individuals. However, we were not able to detect the presence of genomic DNA copies of Streptococcus mutans in any DNA samples extracted from peripheral blood, which suggests the assay may not be sensitive enough for this goal. Values of the threshold cycle of the real-time PCR reaction correlate with higher levels of caries experience in children, but this correlation could not be detected for adults.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Vieira, Alexandre Rarv11@pitt.eduARV11
Deeley, Kathleen B
Callahan, Nicholas F
Noel, Jacqueline B
Anjomshoaa, Ida
Carricato, Wendy M
Schulhof, Louise P
Desensi, Rebecca S
Gandhi, Pooja
Resick, Judith M
Brandon, Carla A
Rozhon, Christopher
Patir, Asli
Yildirim, Mine
Poletta, Fernando A
Mereb, Juan C
Letra, Ariadne
Menezes, Renato
Wendell, Steven
Lopez-Camelo, Jorge S
Castilla, Eduardo E
Orioli, Iêda M
Seymen, Figen
Weyant, Robert Jrjw1@pitt.eduRJW10000-0002-5252-9120
Crout, Richard
McNeil, Daniel W
Modesto, Adriana
Marazita, Mary L
Date: 1 March 2011
Date Type: Acceptance
Journal or Publication Title: ISRN Dent
Volume: 2011
Page Range: 543561 - ?
DOI or Unique Handle: 10.5402/2011/543561
Schools and Programs: School of Dental Medicine > Dental Science
Refereed: Yes
Funders: NCRR NIH HHS (TL1 RR024155-02), NIDCR NIH HHS (R01 DE018914), NCATS NIH HHS (TL1 TR000145), NCRR NIH HHS (TL1 RR024155), NIDCR NIH HHS (R01 DE016148-05), NIDCR NIH HHS (R01 DE018914-03), NIDCR NIH HHS (R01 DE016148-01), NIDCR NIH HHS (P50 DE016215-01), NIDCR NIH HHS (R01 DE016148-06), NCRR NIH HHS (TL1 RR024155-03), NIDCR NIH HHS (R01 DE014899), NIDCR NIH HHS (R01 DE016148-04A1), NIDCR NIH HHS (P50 DE016215-02), NCRR NIH HHS (TL1 RR024155-06), NIDCR NIH HHS (R01 DE018914-02), NIDCR NIH HHS (R01 DE016148-06S1), NIDCR NIH HHS (R01 DE016148), NCRR NIH HHS (TL1 RR024155-04), NIDCR NIH HHS (R01 DE016148-02), NCRR NIH HHS (TL1 RR024155-05), NIDCR NIH HHS (R01 DE016148-03), NIDCR NIH HHS (P50 DE016215-03), NIDCR NIH HHS (R01 DE016148-05S2), NIDCR NIH HHS (P50 DE016215-05), NIDCR NIH HHS (R01 DE016148-07), NIDCR NIH HHS (R01 DE018914-01A1), NIDCR NIH HHS (P50 DE016215-04S1), NIDCR NIH HHS (P50 DE016215-03S1), NIDCR NIH HHS (P50 DE016215-04), NIDCR NIH HHS (R01 DE016148-05S1), NIDCR NIH HHS (P50 DE016215), NCRR NIH HHS (TL1 RR024155-01)
Other ID: NLM NIHMS288582, NLM PMC3128887
PubMed Central ID: PMC3128887
PubMed ID: 21731912
Date Deposited: 24 Sep 2012 20:56
Last Modified: 20 Feb 2020 13:55
URI: http://d-scholarship.pitt.edu/id/eprint/14346

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