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UV resonance Raman study of angiotensin II conformation in nonaqueous environments: lipid micelles and acetonitrile.

Holtz, JS and Lednev, IK and Asher, SA (2000) UV resonance Raman study of angiotensin II conformation in nonaqueous environments: lipid micelles and acetonitrile. Biopolymers, 57 (2). 55 - 63. ISSN 0006-3525

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We used 206.5-nm excited resonance Raman measurements to examine the angiotensin II (AII) secondary structure in H(2)O in the presence of dodecylphosphocholine (DPC) micelles, sodium dodecylsulfate (SDS) monomers and micelles, and in a 70% acetonitrile (ACN-d)-30% water solution. Our AII-SDS titration absorption studies indicate the formation of a 1:2 AII:SDS complex in which two negatively charged SDS molecules attach to the AII positively charged N terminus and to Arg(2). Our 206.5-nm excited Raman results indicate that the 1:2 AII:SDS complexation increases the AII beta-turn composition. We also used 228.9-nm Raman excitation to probe the local solvent accessibility of Tyr(4) (AII) in DPC and SDS micelles. Our Tyr (AII) solvent accessibility studies suggest that the Tyr residue is more exposed to the aqueous environment in SDS micelles than in DPC micelles.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Holtz, JS
Lednev, IK
Asher, SAasher@pitt.eduASHER
Date: 2000
Date Type: Publication
Journal or Publication Title: Biopolymers
Volume: 57
Number: 2
Page Range: 55 - 63
DOI or Unique Handle: 10.1002/(sici)1097-0282(2000)57:2<55::aid-bip2>;2-b
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
Refereed: Yes
Uncontrolled Keywords: Acetonitriles, Angiotensin II, Animals, Humans, Lipids, Membranes, Artificial, Micelles, Phosphorylcholine, Protein Conformation, Protein Structure, Secondary, Sodium Dodecyl Sulfate, Solvents, Spectrum Analysis, Raman, Ultraviolet Rays, Water
ISSN: 0006-3525
Funders: NIGMS NIH HHS (R01GM30741)
MeSH Headings: Acetonitriles--pharmacology; Angiotensin II--chemistry; Angiotensin II--radiation effects; Animals; Humans; Lipids--chemistry; Lipids--pharmacology; Membranes, Artificial; Micelles; Phosphorylcholine--analogs & derivatives; Phosphorylcholine--chemistry; Phosphorylcholine--pharmacology; Protein Conformation--drug effects; Protein Structure, Secondary--drug effects; Sodium Dodecyl Sulfate--chemistry; Sodium Dodecyl Sulfate--pharmacology; Solvents--pharmacology; Spectrum Analysis, Raman--methods; Ultraviolet Rays; Water--pharmacology
Other ID: 10.1002/(SICI)1097-0282(2000)57:2<55::AID-BIP2>3.0.CO;2-B
PubMed ID: 10766956
Date Deposited: 25 Sep 2012 14:22
Last Modified: 02 Dec 2017 16:55


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