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Serratamolide is a hemolytic factor produced by Serratia marcescens

Shanks, RMQ and Stella, NA and Lahr, RM and Wang, S and Veverka, TI and Kowalski, RP and Liu, X (2012) Serratamolide is a hemolytic factor produced by Serratia marcescens. PLoS ONE, 7 (5).

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Abstract

Serratia marcescens is a common contaminant of contact lens cases and lenses. Hemolytic factors of S. marcescens contribute to the virulence of this opportunistic bacterial pathogen. We took advantage of an observed hyper-hemolytic phenotype of crp mutants to investigate mechanisms of hemolysis. A genetic screen revealed that swrW is necessary for the hyper-hemolysis phenotype of crp mutants. The swrW gene is required for biosynthesis of the biosurfactant serratamolide, previously shown to be a broad-spectrum antibiotic and to contribute to swarming motility. Multicopy expression of swrW or mutation of the hexS transcription factor gene, a known inhibitor of swrW expression, led to an increase in hemolysis. Surfactant zones and expression from an swrW-transcriptional reporter were elevated in a crp mutant compared to the wild type. Purified serratamolide was hemolytic to sheep and murine red blood cells and cytotoxic to human airway and corneal limbal epithelial cells in vitro. The swrW gene was found in the majority of contact lens isolates tested. Genetic and biochemical analysis implicate the biosurfactant serratamolide as a hemolysin. This novel hemolysin may contribute to irritation and infections associated with contact lens use. © 2012 Shanks et al.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Shanks, RMQrms68@pitt.eduRMS68
Stella, NAnas92@pitt.eduNAS92
Lahr, RMrml40@pitt.eduRML400000-0002-7469-4565
Wang, S
Veverka, TI
Kowalski, RPkowalsk@pitt.eduKOWALSK0000-0002-8558-0782
Liu, Xxinyuliu@pitt.eduXINYULIU
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorWebber, Mark AlexanderUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 16 May 2012
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 7
Number: 5
DOI or Unique Handle: 10.1371/journal.pone.0036398
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
School of Medicine > Ophthalmology
Refereed: Yes
MeSH Headings: Contact Lenses--microbiology; Depsipeptides--pharmacology; Genes, Bacterial; Genetic Complementation Test; Hemolysis--drug effects; Mutation; Serratia marcescens--genetics; Serratia marcescens--isolation & purification; Serratia marcescens--metabolism
Other ID: NLM PMC3353980
PubMed Central ID: PMC3353980
PubMed ID: 22615766
Date Deposited: 04 Oct 2012 14:14
Last Modified: 04 Feb 2019 15:58
URI: http://d-scholarship.pitt.edu/id/eprint/15568

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