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Requirement for a Uroplakin 3a-like protein in the development of zebrafish pronephric tubule epithelial cell function, morphogenesis, and polarity

Mitra, S and Lukianov, S and Ruiz, WG and Cosentino, C and Sanker, S and Traub, LM and Hukriede, NA and Apodaca, G (2012) Requirement for a Uroplakin 3a-like protein in the development of zebrafish pronephric tubule epithelial cell function, morphogenesis, and polarity. PLoS ONE, 7 (7).

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Abstract

Uroplakin (UP)3a is critical for urinary tract development and function; however, its role in these processes is unknown. We examined the function of the UP3a-like protein Upk3l, which was expressed at the apical surfaces of the epithelial cells that line the pronephric tubules (PTs) of the zebrafish pronephros. Embryos treated with upk3l-targeted morpholinos showed decreased pronephros function, which was attributed to defects in PT epithelial cell morphogenesis and polarization including: loss of an apical brush border and associated phospho-ERM proteins, apical redistribution of the basolateral Na+/K+-ATPase, and altered or diminished expression of the apical polarity complex proteins Prkcz (atypical protein kinase C zeta) and Pard3 (Par3). Upk3l missing its C-terminal cytoplasmic domain or containing mutations in conserved tyrosine or proline residues did not rescue, or only partially rescued the effects of Upk3l depletion. Our studies indicate that Upk3l promotes epithelial polarization and morphogenesis, likely by forming or stimulating interactions with cytoplasmic signaling or polarity proteins, and that defects in this process may underlie the pathology observed in UP3a knockout mice or patients with renal abnormalities that result from altered UP3a expression. © 2012 Mitra et al.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Mitra, S
Lukianov, S
Ruiz, WGruizw@pitt.eduRUIZW
Cosentino, C
Sanker, Ssus48@pitt.eduSUS48
Traub, LMtraub@pitt.eduTRAUB
Hukriede, NAhukriede@pitt.eduHUKRIEDE
Apodaca, Ggla6@pitt.eduGLA6
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorSingh, Shree RamUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 25 July 2012
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 7
Number: 7
DOI or Unique Handle: 10.1371/journal.pone.0041816
Schools and Programs: School of Medicine > Cell Biology
School of Medicine > Developmental Biology
Refereed: Yes
Other ID: NLM PMC3404999
PubMed Central ID: PMC3404999
PubMed ID: 22848617
Date Deposited: 10 Oct 2012 14:03
Last Modified: 26 Jun 2018 02:55
URI: http://d-scholarship.pitt.edu/id/eprint/15597

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