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The synergistic effect of treadmill running on stem-cell transplantation to heal injured skeletal muscle

Ambrosio, F and Ferrari, RJ and Distefano, G and Plassmeyer, JM and Carvell, GE and Deasy, BM and Boninger, ML and Fitzgerald, GK and Huard, J (2010) The synergistic effect of treadmill running on stem-cell transplantation to heal injured skeletal muscle. Tissue Engineering - Part A, 16 (3). 839 - 849. ISSN 1937-3341

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Abstract

Muscle-derived stem-cell (MDSC) transplantation presents a promising method for the treatment of muscle injuries. This study investigated the ability of exercise to enhance MDSC transplantation into the injured muscle. Mice were divided into four groups: contusion+phosphate-buffered saline (C+PBS; n=14 muscles), C+MDSC transplantation (n=12 muscles), C+PBS+treadmill running (C+PBS+TM; n=17 muscles), and C+MDSC+TM (n=13 muscles). One day after injury, the TM groups began running for 1 or 5 weeks. Two days after injury, muscles of C+MDSC and C+MDSC+TM groups were injected with MDSCs. One or 5 weeks later, the number and differentiation of transplanted MDSCs, myofiber regeneration, collagen I formation, and vascularity were assessed histologically. In vitro, MDSCs were subjected to mechanical stimulation, and growth kinetics were quantified. In vitro, mechanical stimulation decreased the MDSC population doubling time (18.6±1.6h) and cell division time (10.9±0.7h), compared with the controls (population doubling time: 23.0±3.4h; cell division time: 13.3±1.1h) (p=0.01 and 0.03, respectively). In vivo, 5 weeks of TM increased the myogenic contribution of transplanted MDSCs, compared with the controls (p=0.02). C+MDSC, C+PBS+TM, and C+MDSC+TM demonstrated decreased fibrosis at 5 weeks, compared with the C+PBS controls (p=0.00, p=0.03, and p=0.02, respectively). Results suggest that the mechanical stimulation favors MDSC proliferation, both in vitro and in vivo, and that exercise enhances MDSC transplantation after injury. © 2010 Mary Ann Liebert, Inc.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Ambrosio, Ffaa7@pitt.eduFAA7
Ferrari, RJ
Distefano, G
Plassmeyer, JM
Carvell, GEgcarvell@pitt.eduGCARVELL
Deasy, BM
Boninger, MLboninger@pitt.eduBONINGER
Fitzgerald, GK
Huard, J
Centers: Other Centers, Institutes, Offices, or Units > Human Engineering Research Laboratories
Date: 1 March 2010
Date Type: Publication
Journal or Publication Title: Tissue Engineering - Part A
Volume: 16
Number: 3
Page Range: 839 - 849
DOI or Unique Handle: 10.1089/ten.tea.2009.0113
Schools and Programs: School of Health and Rehabilitation Sciences > Rehabilitation Science and Technology
Refereed: Yes
ISSN: 1937-3341
MeSH Headings: Animals; Cell Differentiation; Cell Lineage; Cell Movement; Cell Proliferation; Collagen--metabolism; Female; Fluorescent Antibody Technique; Kinetics; Mice; Mice, Inbred C57BL; Muscle Fibers, Skeletal--pathology; Muscle, Skeletal--blood supply; Muscle, Skeletal--pathology; Physical Conditioning, Animal; Regeneration; Stem Cell Transplantation; Transduction, Genetic; Wound Healing; beta-Galactosidase--metabolism
Other ID: NLM PMC2862616
PubMed Central ID: PMC2862616
PubMed ID: 19788347
Date Deposited: 18 Oct 2012 19:04
Last Modified: 04 Feb 2019 16:55
URI: http://d-scholarship.pitt.edu/id/eprint/15959

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