Banaiee, N and Bobadilla-del-Valle, M and Riska, PF and Bardarov, S and Small, PM and Ponce-de-Leon, A and Jacobs, WR and Hatfull, GF and Sifuentes-Osornio, J
(2003)
Rapid identification and susceptibility testing of Mycobacterium tuberculosis from MGIT cultures with luciferase reporter mycobacteriophages.
Journal of Medical Microbiology, 52 (7).
557 - 561.
ISSN 0022-2615
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Abstract
In a prospective study conducted in a diagnostic laboratory in Mexico City, luciferase reporter mycobacteriophages (LRPs) were evaluated for their utility and performance in identification and antibiotic-susceptibility testing of Mycobacterium tuberculosis complex (MTC) isolates from MGIT-960 cultures. Eighty-four consecutive MGIT cultures recovered from 54 patients were included in this study. The LRPs confirmed mycobacterial growth in 79 (94%) of 84 MGIT cultures. Failure to confirm growth was due to low inoculum (n = 1) or growth with non-tuberculous mycobacteria (n = 4). The median time to confirmation of MGIT cultures was 1 day (range 1-55). Confirmed cultures were identified with p-nitro-α-acetylamino-β-hydroxypropiophenone (NAP), a selective inhibitor of MTC species, and results obtained with LRPs were compared with those obtained by BACTEC-460. The sensitivity and specificity of the LRP NAP test were respectively 97 and 100%, and the median turnaround time for identification was 3 days with both methods. The accuracy and speed of the LRPs for susceptibility testing with rifampicin, streptomycin, isoniazid and ethambutol were compared with BACTEC-460 and discrepant results were tested by the conventional agar proportion method. In total, 72 MTC cultures were tested. The overall agreement between the LRPs and BACTEC-460 was 98.6%. Four isolates (5.6%) were falsely identified as ethambutol-resistant. The median turnaround time for susceptibility testing was 3 days (range 3-57) with the LRPs and 9 days (range 7-29) with BACTEC-460. LRPs offer an accurate and rapid approach for identification and susceptibility testing of M. tuberculosis from MGIT-960 cultures.
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Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
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Banaiee, N | | | | Bobadilla-del-Valle, M | | | | Riska, PF | | | | Bardarov, S | | | | Small, PM | | | | Ponce-de-Leon, A | | | | Jacobs, WR | | | | Hatfull, GF | gfh@pitt.edu | GFH | | Sifuentes-Osornio, J | | | |
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Date: |
1 July 2003 |
Date Type: |
Publication |
Journal or Publication Title: |
Journal of Medical Microbiology |
Volume: |
52 |
Number: |
7 |
Page Range: |
557 - 561 |
DOI or Unique Handle: |
10.1099/jmm.0.05149-0 |
Schools and Programs: |
Dietrich School of Arts and Sciences > Biological Sciences |
Refereed: |
Yes |
ISSN: |
0022-2615 |
MeSH Headings: |
Antitubercular Agents--pharmacology; Drug Resistance, Multiple, Bacterial; Genes, Reporter; Humans; Luciferases--genetics; Microbial Sensitivity Tests--methods; Mycobacteriophages--genetics; Mycobacterium tuberculosis--drug effects; Mycobacterium tuberculosis--physiology; Mycobacterium tuberculosis--virology; Tuberculosis--microbiology |
PubMed ID: |
12808076 |
Date Deposited: |
29 Oct 2012 20:24 |
Last Modified: |
02 Feb 2019 15:57 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/16126 |
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