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Rapid identification and susceptibility testing of Mycobacterium tuberculosis from MGIT cultures with luciferase reporter mycobacteriophages

Banaiee, N and Bobadilla-del-Valle, M and Riska, PF and Bardarov, S and Small, PM and Ponce-de-Leon, A and Jacobs, WR and Hatfull, GF and Sifuentes-Osornio, J (2003) Rapid identification and susceptibility testing of Mycobacterium tuberculosis from MGIT cultures with luciferase reporter mycobacteriophages. Journal of Medical Microbiology, 52 (7). 557 - 561. ISSN 0022-2615

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In a prospective study conducted in a diagnostic laboratory in Mexico City, luciferase reporter mycobacteriophages (LRPs) were evaluated for their utility and performance in identification and antibiotic-susceptibility testing of Mycobacterium tuberculosis complex (MTC) isolates from MGIT-960 cultures. Eighty-four consecutive MGIT cultures recovered from 54 patients were included in this study. The LRPs confirmed mycobacterial growth in 79 (94%) of 84 MGIT cultures. Failure to confirm growth was due to low inoculum (n = 1) or growth with non-tuberculous mycobacteria (n = 4). The median time to confirmation of MGIT cultures was 1 day (range 1-55). Confirmed cultures were identified with p-nitro-α-acetylamino-β-hydroxypropiophenone (NAP), a selective inhibitor of MTC species, and results obtained with LRPs were compared with those obtained by BACTEC-460. The sensitivity and specificity of the LRP NAP test were respectively 97 and 100%, and the median turnaround time for identification was 3 days with both methods. The accuracy and speed of the LRPs for susceptibility testing with rifampicin, streptomycin, isoniazid and ethambutol were compared with BACTEC-460 and discrepant results were tested by the conventional agar proportion method. In total, 72 MTC cultures were tested. The overall agreement between the LRPs and BACTEC-460 was 98.6%. Four isolates (5.6%) were falsely identified as ethambutol-resistant. The median turnaround time for susceptibility testing was 3 days (range 3-57) with the LRPs and 9 days (range 7-29) with BACTEC-460. LRPs offer an accurate and rapid approach for identification and susceptibility testing of M. tuberculosis from MGIT-960 cultures.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Banaiee, N
Bobadilla-del-Valle, M
Riska, PF
Bardarov, S
Small, PM
Ponce-de-Leon, A
Jacobs, WR
Hatfull, GFgfh@pitt.eduGFH
Sifuentes-Osornio, J
Date: 1 July 2003
Date Type: Publication
Journal or Publication Title: Journal of Medical Microbiology
Volume: 52
Number: 7
Page Range: 557 - 561
DOI or Unique Handle: 10.1099/jmm.0.05149-0
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0022-2615
MeSH Headings: Antitubercular Agents--pharmacology; Drug Resistance, Multiple, Bacterial; Genes, Reporter; Humans; Luciferases--genetics; Microbial Sensitivity Tests--methods; Mycobacteriophages--genetics; Mycobacterium tuberculosis--drug effects; Mycobacterium tuberculosis--physiology; Mycobacterium tuberculosis--virology; Tuberculosis--microbiology
PubMed ID: 12808076
Date Deposited: 29 Oct 2012 20:24
Last Modified: 02 Feb 2019 15:57


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