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Integration and excision by the large serine recombinase φRv1 integrase

Bibb, LA and Hancox, MI and Hatfull, GF (2005) Integration and excision by the large serine recombinase φRv1 integrase. Molecular Microbiology, 55 (6). 1896 - 1910. ISSN 0950-382X

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The Mycobacterium tuberculosis prophage-like element φRv1 encodes a site-specific recombination system utilizing an integrase of the serine recombinase family. Recombination occurs between a putative attP site and the host chromosome, but is unusual in that the attB site lies within a redundant repetitive element (REP13E12) of which there are seven copies in the M. tuberculosis genome; four of these elements contain attB sites suitable for φRv1 integration in vivo. Although the mechanism of directional control of large serine integrases is poorly understood, a recombination directionality factor (RDF) has been identified that is required for φRv1 integrase-mediated excisive recombination in vivo. Here we describe defined in vitro recombination reactions for both φRv1 integrase-mediated integration and excision and show that the φRv1 RDF is not only required for excision but inhibits integrative recombination; neither reaction requires DNA supercoiling, host factors, or high-energy cofactors. Integration, excision and excise-mediated inhibition of integration require simple substrates sites, indicating that the control of directionality does not involve the manipulation of higher-order protein-DNA architectures as described for the tyrosine integrases. © 2005 Blackwell Publishing Ltd.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Bibb, LA
Hancox, MI
Hatfull, GFgfh@pitt.eduGFH
Date: 1 March 2005
Date Type: Publication
Journal or Publication Title: Molecular Microbiology
Volume: 55
Number: 6
Page Range: 1896 - 1910
DOI or Unique Handle: 10.1111/j.1365-2958.2005.04517.x
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0950-382X
MeSH Headings: Attachment Sites, Microbiological--genetics; Attachment Sites, Microbiological--physiology; Base Sequence; DNA, Bacterial--metabolism; DNA, Viral--metabolism; Integrases--physiology; Molecular Sequence Data; Mycobacteriophages--enzymology; Mycobacteriophages--genetics; Mycobacteriophages--physiology; Mycobacterium tuberculosis--virology; Prophages--enzymology; Prophages--genetics; Prophages--physiology; Recombination, Genetic; Substrate Specificity; Virus Integration
PubMed ID: 15752208
Date Deposited: 12 Nov 2012 15:52
Last Modified: 02 Feb 2019 15:57


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