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Integration and Excision of the Mycobacterium Tuberculosis Prophage-Like Element, PhiRv1.

Bibb, Lori A and Hatfull, Graham F (2002) Integration and Excision of the Mycobacterium Tuberculosis Prophage-Like Element, PhiRv1. Molecular microbiology, 45 (6). 1515 - 1526. ISSN 0950-382X

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The genomes of Mycobacterium tuberculosis H37Rv and CDC1551 each contain two prophage-like elements, phiRv1 and phiRv2. The phiRv1 element is not only absent from Mycobacterium bovis BCG but is in different locations within the two sequenced M. tuberculosis genomes; in both cases phiRv1 is inserted into a REP13E12 repeated sequence, which presumably contains the bacterial attachment site, attB, for phiRv1. Although phiRv1 is probably too small to encode infectious phage particles, it may nevertheless have an active integration/excision system and be capable of moving from one chromosomal position to another. We show here that the M. tuberculosis H37Rv phiRv1 element does indeed encode an active site-specific recombination system in which an integrase of the serine recombinase family (Rv1586c) catalyses integration and excision and a small, basic phiRv1-encoded protein (Rv1584c) controls the directionality of re-combination. Integration-proficient plasmid vectors derived from phiRv1 efficiently transform BCG, can utilize four of the seven REP13E12 sites present in BCG as attachment sites, and can occupy more than one site simultaneously.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Bibb, Lori A
Hatfull, Graham Fgfh@pitt.eduGFH
Date: 15 September 2002
Date Type: Publication
Journal or Publication Title: Molecular microbiology
Volume: 45
Number: 6
Publisher: Blackwell Publ., Ltd
Page Range: 1515 - 1526
DOI or Unique Handle: 10.1046/j.1365-2958.2002.03130.x/pdf
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0950-382X
Article Type: Research Article
MeSH Headings: Amino Acid Sequence; Attachment Sites, Microbiological; Base Sequence; DNA Transposable Elements; Molecular Sequence Data; Mycobacteriophages--genetics; Mycobacteriophages--physiology; Mycobacterium smegmatis--genetics; Mycobacterium tuberculosis--virology; Plasmids--genetics; Prophages--genetics; Prophages--physiology; Recombination, Genetic; Transformation, Bacterial; Virus Integration
PubMed ID: 12354222
Date Deposited: 12 Nov 2012 15:51
Last Modified: 19 Mar 2021 04:55


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