Casjens, Sherwood R and Gilcrease, Eddie B and Huang, Wai Mun and Bunny, Kim L and Pedulla, Marisa L and Ford, Michael E and Houtz, Jennifer M and Hatfull, Graham F and Hendrix, Roger W
(2004)
The PKO2 Linear Plasmid Prophage of Klebsiella Oxytoca.
Journal of bacteriology, 186 (6).
1818 - 1832.
ISSN 0021-9193
Abstract
Temperate bacteriophages with plasmid prophages are uncommon in nature, and of these only phages N15 and PY54 are known to have a linear plasmid prophage with closed hairpin telomeres. We report here the complete nucleotide sequence of the 51,601-bp Klebsiella oxytoca linear plasmid pKO2, and we demonstrate experimentally that it is also a prophage. We call this bacteriophage phiKO2. An analysis of the 64 predicted phiKO2 genes indicate that it is a fairly close relative of phage N15; they share a mosaic relationship that is typical of different members of double-stranded DNA tailed-phage groups. Although the head, tail shaft, and lysis genes are not recognizably homologous between these phages, other genes such as the plasmid partitioning, replicase, prophage repressor, and protelomerase genes (and their putative targets) are so similar that we predict that they must have nearly identical DNA binding specificities. The phiKO2 virion is unusual in that its phage lambda-like tails have an exceptionally long (3,433 amino acids) central tip tail fiber protein. The phiKO2 genome also carries putative homologues of bacterial dinI and umuD genes, both of which are involved in the host SOS response. We show that these divergently transcribed genes are regulated by LexA protein binding to a single target site that overlaps both promoters.
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| Item Type: |
Article
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| Status: |
Published |
| Creators/Authors: |
| Creators | Email | Pitt Username | ORCID  |
|---|
| Casjens, Sherwood R | | | | | Gilcrease, Eddie B | | | | | Huang, Wai Mun | | | | | Bunny, Kim L | | | | | Pedulla, Marisa L | | | | | Ford, Michael E | | | | | Houtz, Jennifer M | | | | | Hatfull, Graham F | gfh@pitt.edu | GFH | | | Hendrix, Roger W | | | |
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| Date: |
March 2004 |
| Date Type: |
Publication |
| Journal or Publication Title: |
Journal of bacteriology |
| Volume: |
186 |
| Number: |
6 |
| Publisher: |
American Society for Microbiology |
| Page Range: |
1818 - 1832 |
| DOI or Unique Handle: |
10.1128/jb.186.6.1818-1832.2004 |
| Schools and Programs: |
Dietrich School of Arts and Sciences > Biological Sciences |
| Refereed: |
Yes |
| ISSN: |
0021-9193 |
| Article Type: |
Research Article |
| MeSH Headings: |
Amino Acid Sequence; Bacteriophages--genetics; Bacteriophages--metabolism; DNA--metabolism; Gene Expression Regulation, Viral; Klebsiella oxytoca--genetics; Klebsiella oxytoca--virology; Molecular Sequence Data; Plasmids--genetics; Prophages--genetics; Prophages--metabolism; Sequence Analysis, DNA; Transcription, Genetic; Viral Proteins--genetics; Viral Proteins--metabolism; Virion--metabolism; Virion--ultrastructure |
| Other ID: |
NLM PMC355964 |
| PubMed Central ID: |
PMC355964 |
| PubMed ID: |
14996813 |
| Date Deposited: |
12 Nov 2012 15:51 |
| Last Modified: |
27 Sep 2022 15:19 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/16194 |
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