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The PKO2 Linear Plasmid Prophage of Klebsiella Oxytoca.

Casjens, Sherwood R and Gilcrease, Eddie B and Huang, Wai Mun and Bunny, Kim L and Pedulla, Marisa L and Ford, Michael E and Houtz, Jennifer M and Hatfull, Graham F and Hendrix, Roger W (2004) The PKO2 Linear Plasmid Prophage of Klebsiella Oxytoca. Journal of bacteriology, 186 (6). 1818 - 1832. ISSN 0021-9193

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Temperate bacteriophages with plasmid prophages are uncommon in nature, and of these only phages N15 and PY54 are known to have a linear plasmid prophage with closed hairpin telomeres. We report here the complete nucleotide sequence of the 51,601-bp Klebsiella oxytoca linear plasmid pKO2, and we demonstrate experimentally that it is also a prophage. We call this bacteriophage phiKO2. An analysis of the 64 predicted phiKO2 genes indicate that it is a fairly close relative of phage N15; they share a mosaic relationship that is typical of different members of double-stranded DNA tailed-phage groups. Although the head, tail shaft, and lysis genes are not recognizably homologous between these phages, other genes such as the plasmid partitioning, replicase, prophage repressor, and protelomerase genes (and their putative targets) are so similar that we predict that they must have nearly identical DNA binding specificities. The phiKO2 virion is unusual in that its phage lambda-like tails have an exceptionally long (3,433 amino acids) central tip tail fiber protein. The phiKO2 genome also carries putative homologues of bacterial dinI and umuD genes, both of which are involved in the host SOS response. We show that these divergently transcribed genes are regulated by LexA protein binding to a single target site that overlaps both promoters.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Casjens, Sherwood R
Gilcrease, Eddie B
Huang, Wai Mun
Bunny, Kim L
Pedulla, Marisa L
Ford, Michael E
Houtz, Jennifer M
Hatfull, Graham Fgfh@pitt.eduGFH
Hendrix, Roger W
Date: March 2004
Date Type: Publication
Journal or Publication Title: Journal of bacteriology
Volume: 186
Number: 6
Publisher: American Society for Microbiology
Page Range: 1818 - 1832
DOI or Unique Handle: 10.1128/​jb.186.6.1818-1832.2004
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0021-9193
Article Type: Research Article
MeSH Headings: Amino Acid Sequence; Bacteriophages--genetics; Bacteriophages--metabolism; DNA--metabolism; Gene Expression Regulation, Viral; Klebsiella oxytoca--genetics; Klebsiella oxytoca--virology; Molecular Sequence Data; Plasmids--genetics; Prophages--genetics; Prophages--metabolism; Sequence Analysis, DNA; Transcription, Genetic; Viral Proteins--genetics; Viral Proteins--metabolism; Virion--metabolism; Virion--ultrastructure
Other ID: NLM PMC355964
PubMed Central ID: PMC355964
PubMed ID: 14996813
Date Deposited: 12 Nov 2012 15:51
Last Modified: 31 Jul 2020 19:02


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