Casjens, SR and Gilcrease, EB and Winn-Stapley, DA and Schicklmaier, P and Schmieger, H and Pedulla, ML and Ford, ME and Houtz, JM and Hatfull, GF and Hendrix, RW
(2005)
The generalized transducing Salmonella bacteriophage ES18: Complete genome sequence and DNA packaging strategy.
Journal of Bacteriology, 187 (3).
1091 - 1104.
ISSN 0021-9193
Abstract
The generalized transducing double-stranded DNA bacteriophage ES18 has an icosahedral head and a long noncontractile tail, and it infects both rough and smooth Salmonella enterica strains. We report here the complete 46,900-bp genome nucleotide sequence and provide an analysis of the sequence. Its 79 genes and their organization clearly show that ES18 is a member of the lambda-like (lambdoid) phage group; however, it contains a novel set of genes that program assembly of the virion head. Most of its integration-excision, immunity, Nin region, and lysis genes are nearly identical to those of the short-tailed Salmonella phage P22, while other early genes are nearly identical to Escherichia coli phages λ and HK97, S. enterica phage ST64T, or a Shigella flexneri prophage. Some of the ES18 late genes are novel, while others are most closely related to phages HK97, lambda, or N15. Thus, the ES18 genome is mosaically related to other lambdoid phages, as is typical for all group members. Analysis of virion DNA showed that it is circularly permuted and about 10% terminally redundant and that initiation of DNA packaging series occurs across an approximately 1-kbp region rather than at a precise location on the genome. This supports a model in which ES18 terminase can move substantial distances along the DNA between recognition and cleavage of DNA destined to be packaged. Bio-informatic analysis of large terminase subunits shows that the different functional classes of phage-encoded terminases can usually be predicted from their amino acid sequence.
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| Item Type: |
Article
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| Status: |
Published |
| Creators/Authors: |
| Creators | Email | Pitt Username | ORCID  |
|---|
| Casjens, SR | | | | | Gilcrease, EB | | | | | Winn-Stapley, DA | | | | | Schicklmaier, P | | | | | Schmieger, H | | | | | Pedulla, ML | | | | | Ford, ME | | | | | Houtz, JM | | | | | Hatfull, GF | gfh@pitt.edu | GFH | | | Hendrix, RW | rhx@pitt.edu | RHX | |
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| Date: |
1 February 2005 |
| Date Type: |
Publication |
| Journal or Publication Title: |
Journal of Bacteriology |
| Volume: |
187 |
| Number: |
3 |
| Page Range: |
1091 - 1104 |
| DOI or Unique Handle: |
10.1128/jb.187.3.1091-1104.2005 |
| Schools and Programs: |
Dietrich School of Arts and Sciences > Biological Sciences |
| Refereed: |
Yes |
| ISSN: |
0021-9193 |
| MeSH Headings: |
Base Sequence; DNA Replication; DNA, Viral--genetics; Genome, Viral; Microscopy, Electron; Salmonella--virology; Salmonella Phages--genetics; Salmonella Phages--ultrastructure; Viral Proteins--genetics; Virion--ultrastructure |
| Other ID: |
NLM PMC545730 |
| PubMed Central ID: |
PMC545730 |
| PubMed ID: |
15659686 |
| Date Deposited: |
12 Nov 2012 15:50 |
| Last Modified: |
28 Sep 2022 13:11 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/16195 |
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