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The generalized transducing Salmonella bacteriophage ES18: Complete genome sequence and DNA packaging strategy

Casjens, SR and Gilcrease, EB and Winn-Stapley, DA and Schicklmaier, P and Schmieger, H and Pedulla, ML and Ford, ME and Houtz, JM and Hatfull, GF and Hendrix, RW (2005) The generalized transducing Salmonella bacteriophage ES18: Complete genome sequence and DNA packaging strategy. Journal of Bacteriology, 187 (3). 1091 - 1104. ISSN 0021-9193

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Abstract

The generalized transducing double-stranded DNA bacteriophage ES18 has an icosahedral head and a long noncontractile tail, and it infects both rough and smooth Salmonella enterica strains. We report here the complete 46,900-bp genome nucleotide sequence and provide an analysis of the sequence. Its 79 genes and their organization clearly show that ES18 is a member of the lambda-like (lambdoid) phage group; however, it contains a novel set of genes that program assembly of the virion head. Most of its integration-excision, immunity, Nin region, and lysis genes are nearly identical to those of the short-tailed Salmonella phage P22, while other early genes are nearly identical to Escherichia coli phages λ and HK97, S. enterica phage ST64T, or a Shigella flexneri prophage. Some of the ES18 late genes are novel, while others are most closely related to phages HK97, lambda, or N15. Thus, the ES18 genome is mosaically related to other lambdoid phages, as is typical for all group members. Analysis of virion DNA showed that it is circularly permuted and about 10% terminally redundant and that initiation of DNA packaging series occurs across an approximately 1-kbp region rather than at a precise location on the genome. This supports a model in which ES18 terminase can move substantial distances along the DNA between recognition and cleavage of DNA destined to be packaged. Bio-informatic analysis of large terminase subunits shows that the different functional classes of phage-encoded terminases can usually be predicted from their amino acid sequence.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Casjens, SR
Gilcrease, EB
Winn-Stapley, DA
Schicklmaier, P
Schmieger, H
Pedulla, ML
Ford, ME
Houtz, JM
Hatfull, GFgfh@pitt.eduGFH
Hendrix, RWrhx@pitt.eduRHX
Date: 1 February 2005
Date Type: Publication
Journal or Publication Title: Journal of Bacteriology
Volume: 187
Number: 3
Page Range: 1091 - 1104
DOI or Unique Handle: 10.1128/jb.187.3.1091-1104.2005
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0021-9193
MeSH Headings: Base Sequence; DNA Replication; DNA, Viral--genetics; Genome, Viral; Microscopy, Electron; Salmonella--virology; Salmonella Phages--genetics; Salmonella Phages--ultrastructure; Viral Proteins--genetics; Virion--ultrastructure
Other ID: NLM PMC545730
PubMed Central ID: PMC545730
PubMed ID: 15659686
Date Deposited: 12 Nov 2012 15:50
Last Modified: 02 Feb 2019 15:58
URI: http://d-scholarship.pitt.edu/id/eprint/16195

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