Two-step site selection for serine-integrase-mediated excision: DNA-directed integrase conformation and central dinucleotide proofreading

Ghosh, P and Bibb, LA and Hatfull, GF (2008) Two-step site selection for serine-integrase-mediated excision: DNA-directed integrase conformation and central dinucleotide proofreading. Proceedings of the National Academy of Sciences of the United States of America, 105 (9). 3238 - 3243. ISSN 0027-8424

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Abstract

Bacteriophage-encoded serine-integrases are members of the large family of serine-recombinases and catalyze site-specific integrative recombination between a phage attP site and a bacterial attB site to form an integrated prophage. Prophage excision involves a second site-specific recombination event, in which the sites generated by integration, attL and attR, are used as substrates to regenerate attP and attB. Excision is catalyzed by integrase but also requires a phage-encoded recombination directionality factor (RDF). The Bxb1 recombination sites, attP and attB, are small (<50 bp), different in sequence, and quasi-symmetrical, and they give rise to attL- and attR-recombinant products that are asymmetric but similar to each other, each being composed of B- and P-type half-sites. We show here that the determination of correct excision products is a two-step process, with a presynaptic RDF-dependent step that aligns attL and attR in the correct orientation and a postsynaptic step in which the nonpalindromic central dinucleotide confers identity to attL and attR and prevents each from recombining with itself. © 2008 by The National Academy of Sciences of the USA.

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Details

Item Type:	Article
Status:	Published
Creators/Authors:	Creators Email Pitt Username ORCID Ghosh, P Bibb, LA Hatfull, GF gfh@pitt.edu GFH
Date:	4 March 2008
Date Type:	Publication
Journal or Publication Title:	Proceedings of the National Academy of Sciences of the United States of America
Volume:	105
Number:	9
Page Range:	3238 - 3243
DOI or Unique Handle:	10.1073/pnas.0711649105
Schools and Programs:	Dietrich School of Arts and Sciences > Biological Sciences
Refereed:	Yes
ISSN:	0027-8424
MeSH Headings:	Attachment Sites, Microbiological; DNA; Integrases--chemistry; Integrases--metabolism; Mycobacteriophages--enzymology; Nucleotides; Protein Conformation; Recombination, Genetic
Other ID:	NLM PMC2265153
PubMed Central ID:	PMC2265153
PubMed ID:	18299577
Date Deposited:	15 Nov 2012 19:24
Last Modified:	02 Feb 2019 15:57
URI:	http://d-scholarship.pitt.edu/id/eprint/16222

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