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The orientation of mycobacteriophage Bxb1 integration is solely dependent on the central dinucleotide of attP and attB

Ghosh, P and Kim, AI and Hatfull, GF (2003) The orientation of mycobacteriophage Bxb1 integration is solely dependent on the central dinucleotide of attP and attB. Molecular Cell, 12 (5). 1101 - 1111. ISSN 1097-2765

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Abstract

Integration of the mycobacteriophage Bxb1 genome into its host chromosome is catalyzed by a serine-integrase, a member of the transposon-resolvase family of site-specific recombinases. These enzymes use a concerted mechanism of strand exchange involving double-stranded cleavages with two-base extensions, and covalent protein-DNA linkages via phosphoserine bonds. In contrast to the resolvase/invertase recombination systems - where there are strict requirements for a specific synaptic complex within which the catalytic potential of the enzyme is activated - synapsis of attP and attB by Bxb1 integrase is completely promiscuous, aligning the sites with equal proclivity in parallel and antiparallel alignments. Moreover, the catalytic potential of Bxb1 integrase is fully active in either alignment. As a consequence, the nonpalindomic central dinucleotide (5′-GT) at the center of attP and attB is the sole determinant of Bxb1 prophage orientation, and a single base pair substitution in the two sites is sufficient to eliminate orientation control.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Ghosh, P
Kim, AI
Hatfull, GFgfh@pitt.eduGFH
Date: 1 January 2003
Date Type: Publication
Journal or Publication Title: Molecular Cell
Volume: 12
Number: 5
Page Range: 1101 - 1111
DOI or Unique Handle: 10.1016/s1097-2765(03)00444-1
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 1097-2765
MeSH Headings: Attachment Sites, Microbiological; Base Pairing; Base Sequence; DNA Topoisomerases--metabolism; DNA, Viral--genetics; DNA, Viral--metabolism; Integrases--metabolism; Lysogeny; Mycobacteriophages--genetics; Mycobacteriophages--metabolism; Recombination, Genetic; Viral Proteins--metabolism
PubMed ID: 14636570
Date Deposited: 15 Nov 2012 20:21
Last Modified: 02 Feb 2019 15:57
URI: http://d-scholarship.pitt.edu/id/eprint/16223

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