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Diabetes-induced Alternations in Biomechanical Properties of Urinary Bladder Wall in Rats

Wang, CC and Nagatomi, J and Toosi, KK and Yoshimura, N and Hsieh, JH and Chancellor, MB and Sacks, MS (2009) Diabetes-induced Alternations in Biomechanical Properties of Urinary Bladder Wall in Rats. Urology, 73 (4). 911 - 915. ISSN 0090-4295

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Abstract

Objectives: To determine whether diabetes mellitus and the associated changes in bladder function will trigger bladder wall tissue remodeling and concomitant alterations in the mechanical properties. We investigated the time course of changes in function and mechanical properties of diabetic and diuretic rat bladders using both in vivo and in vitro techniques. Methods: Cystometry was performed at 2, 4, and 8 weeks on female Sprague-Dawley rats that had received either a single injection of streptozotocin (65 mg/kg intraperitoneally) or 5% sucrose in drinking water for the duration of the experiments. At each point, the biaxial mechanical properties of 10 × 10-mm tissue specimens obtained from the posterior part of bladder wall were quantified. The changes in overall tissue compliance and mechanical anisotropy as a function of time were examined. Results: Both diabetic and diuretic conditions led to increases in bladder weight, bladder capacity, and in vivo compliance compared with the controls at all points tested. Under biaxial loading, all bladder wall tissues exhibited a nonlinear stress-strain relationship and mechanical anisotropy, with greater tissue compliance in the circumferential direction than in the longitudinal direction. Although the compliance of the bladder wall increased progressively and synchronously in both diabetic and diuretic bladders for ≤4 weeks, only the diabetic bladders continued to increase the compliance for ≤8 weeks (diabetic 0.64 ± 0.04 vs diuretic 0.48 ± 0.05, P = .03). Conclusions: The results of our study have shown that diuresis mainly contributes to the "early" changes of mechanical properties of the bladder, with diabetes inducing additional "late" changes of mechanical properties of the rat bladders after 4 weeks. © 2009 Elsevier Inc. All rights reserved.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Wang, CC
Nagatomi, J
Toosi, KKkkt6@pitt.eduKKT6
Yoshimura, Nnyos@pitt.eduNYOS
Hsieh, JH
Chancellor, MB
Sacks, MS
Centers: Other Centers, Institutes, or Units > Human Engineering Research Laboratories
Date: 1 April 2009
Date Type: Publication
Journal or Publication Title: Urology
Volume: 73
Number: 4
Page Range: 911 - 915
DOI or Unique Handle: 10.1016/j.urology.2008.11.026
Schools and Programs: School of Health and Rehabilitation Sciences > Rehabilitation Science and Technology
School of Medicine > Urology
Swanson School of Engineering > Bioengineering
Refereed: Yes
ISSN: 0090-4295
MeSH Headings: Animals; Biomechanics; Diabetes Mellitus, Experimental--physiopathology; Female; Rats; Rats, Sprague-Dawley; Urinary Bladder--physiopathology
Other ID: NLM NIHMS107624, NLM PMC2728502
PubMed Central ID: PMC2728502
PubMed ID: 19168204
Date Deposited: 02 Nov 2012 21:07
Last Modified: 29 Jan 2019 15:55
URI: http://d-scholarship.pitt.edu/id/eprint/16228

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