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Quantification of bladder smooth muscle orientation in normal and spinal cord injured rats

Nagatomi, J and Toosi, KK and Grashow, JS and Chancellor, MB and Sacks, MS (2005) Quantification of bladder smooth muscle orientation in normal and spinal cord injured rats. Annals of Biomedical Engineering, 33 (8). 1078 - 1089. ISSN 0090-6964

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Abstract

Spinal cord injuries (SCI) often lead to severe bladder dysfunctions. Our previous studies have demonstrated that following SCI, rat bladder wall tissue became hypertrophied, significantly more compliant, and changed its mechanical behavior from orthotropic to isotropic. In order to elucidate the link between the tissue microstructure and mechanical properties of the wall, we have developed a novel semi-automated image analysis method to quantify smooth muscle bundle orientation and mass fraction in the bladder wall tissues from normal and 10 day-post-SCI rats. Results of the present study revealed that there were significant (p < 0.05) increases in smooth muscle area fractions as well as significantly (p < 0.001) fewer cell nuclei per muscle area in the SCI groups compared to the normal groups. Furthermore, while the normal rat bladders exhibited predominant smooth muscle orientation only in the longitudinal direction, the SCI rat bladders exhibited smooth muscles oriented in both the circumferential and longitudinal directions. These results provide first evidence that bladder smooth muscle cells exhibit hypertrophy rather than hyperplasia and developed a second, orthogonal orientation of smooth muscle bundles following SCI. The results of the present study corroborate our previous mechanical anisotropy data and provide the basis for development of structure-based constitutive models for urinary bladder wall tissue. © 2005 Biomedical Engineering Society.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Nagatomi, J
Toosi, KKkkt6@pitt.eduKKT6
Grashow, JS
Chancellor, MB
Sacks, MS
Centers: Other Centers, Institutes, or Units > Human Engineering Research Laboratories
Other Centers, Institutes, or Units > McGowan Institute for Regenerative Medicine
Date: 1 November 2005
Date Type: Publication
Journal or Publication Title: Annals of Biomedical Engineering
Volume: 33
Number: 8
Page Range: 1078 - 1089
DOI or Unique Handle: 10.1007/s10439-005-5776-x
Schools and Programs: School of Health and Rehabilitation Sciences > Rehabilitation Science and Technology
School of Medicine > Urology
Swanson School of Engineering > Bioengineering
Refereed: Yes
ISSN: 0090-6964
MeSH Headings: Animals; Cell Nucleus--metabolism; Cell Nucleus--pathology; Female; Hypertrophy; Muscle, Smooth--pathology; Muscle, Smooth--physiopathology; Rats; Rats, Sprague-Dawley; Spinal Cord Injuries--complications; Spinal Cord Injuries--pathology; Spinal Cord Injuries--physiopathology; Urinary Bladder--pathology; Urinary Bladder--physiopathology; Urinary Bladder, Neurogenic--etiology; Urinary Bladder, Neurogenic--pathology; Urinary Bladder, Neurogenic--physiopathology
PubMed ID: 16133916
Date Deposited: 02 Nov 2012 21:07
Last Modified: 02 Feb 2019 14:55
URI: http://d-scholarship.pitt.edu/id/eprint/16231

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