Jain, S and Hatfull, GF
(2000)
Transcriptional regulation and immunity in mycobacteriophage Bxb1.
Molecular Microbiology, 38 (5).
971 - 985.
ISSN 0950-382X
![[img]](http://d-scholarship.pitt.edu/style/images/fileicons/text_plain.png) |
Plain Text (licence)
Available under License : See the attached license file.
Download (1kB)
|
Abstract
Mycobacteriophage Bxb1 is a temperate phage of Mycobacterium smegmatis that shares a similar genome organization to mycobacteriophage L5, although the two phages are heteroimmune. We have investigated the regulatory circuitry of Bxb1 and found that it encodes a repressor, gp69, which regulates at least two promoters, an early lytic promoter, Pleft, and the divergent promoter, Pright. Bxb1 gp69 is 41% identical to the L5 repressor (gp71) and binds to repressor binding sites that conform to a similar, but distinct, 13 bp asymmetric consensus sequence to that for the L5 gp71 binding sites. The two phage repressors have a strong preference for their cognate binding sites, thus accounting for their immunity phenotypes. The Bxb1 genome contains 34 putative repressor binding sites located throughout the genome, but situated within short intergenic spaces and orientated in only one direction relative to the direction of transcription. Comparison with the locations of repressor binding sites within the L5 genome provides insights into how these unusual regulatory systems evolve.
Share
Citation/Export: |
|
Social Networking: |
|
Details
Item Type: |
Article
|
Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
---|
Jain, S | | | | Hatfull, GF | gfh@pitt.edu | GFH | |
|
Date: |
1 December 2000 |
Date Type: |
Publication |
Journal or Publication Title: |
Molecular Microbiology |
Volume: |
38 |
Number: |
5 |
Page Range: |
971 - 985 |
DOI or Unique Handle: |
10.1046/j.1365-2958.2000.02184.x |
Schools and Programs: |
Dietrich School of Arts and Sciences > Biological Sciences |
Refereed: |
Yes |
ISSN: |
0950-382X |
MeSH Headings: |
Amino Acid Sequence; Base Sequence; DNA, Recombinant; Gene Expression Regulation, Viral; Molecular Sequence Data; Mycobacteriophages--genetics; Mycobacteriophages--immunology; Mycobacterium smegmatis--virology; Promoter Regions, Genetic; Repressor Proteins--chemistry; Repressor Proteins--genetics; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Transcription, Genetic; Viral Proteins--chemistry; Viral Proteins--genetics |
PubMed ID: |
11123672 |
Date Deposited: |
29 Nov 2012 21:01 |
Last Modified: |
22 Jun 2021 12:56 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/16302 |
Metrics
Monthly Views for the past 3 years
Plum Analytics
Altmetric.com
Actions (login required)
 |
View Item |