Lee, S and Kriakov, J and Vilcheze, C and Dai, Z and Hatfull, GF and Jacobs, WR
(2004)
Bxz1, a new generalized transducing phage for mycobacteria.
FEMS Microbiology Letters, 241 (2).
271 - 276.
ISSN 0378-1097
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Abstract
We have isolated and characterized a new generalized transducing phage, Bxz1, from soil sampling at a neighboring Wildlife Preservation Park. The hosts of the phage, measured by the formation of plaques, include fast growing Mycobacterium smegmatis and Mycobacterium vaccae. Bxz1 is capable of transducing chromosomal markers, point mutations, and plasmids at frequencies ranging from 10 -8 to 10 -6 per plaque forming unit between strains of M. smegmatis. We also demonstrated cotransduction of a transposon insertion linked to a point mutation of the ndh gene. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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Details
Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
---|
Lee, S | | | | Kriakov, J | | | | Vilcheze, C | | | | Dai, Z | | | | Hatfull, GF | gfh@pitt.edu | GFH | | Jacobs, WR | | | |
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Date: |
15 December 2004 |
Date Type: |
Publication |
Journal or Publication Title: |
FEMS Microbiology Letters |
Volume: |
241 |
Number: |
2 |
Page Range: |
271 - 276 |
DOI or Unique Handle: |
10.1016/j.femsle.2004.10.032 |
Schools and Programs: |
Dietrich School of Arts and Sciences > Biological Sciences |
Refereed: |
Yes |
ISSN: |
0378-1097 |
MeSH Headings: |
Conjugation, Genetic; Genetic Markers; Mycobacteriophages--genetics; Mycobacteriophages--isolation & purification; Mycobacteriophages--physiology; Mycobacterium--genetics; Mycobacterium--virology; Mycobacterium smegmatis--genetics; Mycobacterium smegmatis--virology; Plasmids; Soil Microbiology; Transduction, Genetic |
PubMed ID: |
15598543 |
Date Deposited: |
29 Nov 2012 21:02 |
Last Modified: |
05 Feb 2019 05:55 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/16350 |
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