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Bxz1, a new generalized transducing phage for mycobacteria

Lee, S and Kriakov, J and Vilcheze, C and Dai, Z and Hatfull, GF and Jacobs, WR (2004) Bxz1, a new generalized transducing phage for mycobacteria. FEMS Microbiology Letters, 241 (2). 271 - 276. ISSN 0378-1097

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Abstract

We have isolated and characterized a new generalized transducing phage, Bxz1, from soil sampling at a neighboring Wildlife Preservation Park. The hosts of the phage, measured by the formation of plaques, include fast growing Mycobacterium smegmatis and Mycobacterium vaccae. Bxz1 is capable of transducing chromosomal markers, point mutations, and plasmids at frequencies ranging from 10 -8 to 10 -6 per plaque forming unit between strains of M. smegmatis. We also demonstrated cotransduction of a transposon insertion linked to a point mutation of the ndh gene. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Lee, S
Kriakov, J
Vilcheze, C
Dai, Z
Hatfull, GFgfh@pitt.eduGFH
Jacobs, WR
Date: 15 December 2004
Date Type: Publication
Journal or Publication Title: FEMS Microbiology Letters
Volume: 241
Number: 2
Page Range: 271 - 276
DOI or Unique Handle: 10.1016/j.femsle.2004.10.032
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0378-1097
MeSH Headings: Conjugation, Genetic; Genetic Markers; Mycobacteriophages--genetics; Mycobacteriophages--isolation & purification; Mycobacteriophages--physiology; Mycobacterium--genetics; Mycobacterium--virology; Mycobacterium smegmatis--genetics; Mycobacterium smegmatis--virology; Plasmids; Soil Microbiology; Transduction, Genetic
PubMed ID: 15598543
Date Deposited: 29 Nov 2012 21:02
Last Modified: 05 Feb 2019 05:55
URI: http://d-scholarship.pitt.edu/id/eprint/16350

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