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Scaffoldless tissue engineering: a model to study tissue formation and a device for tissue regeneration

Syed-Picard, Fatima N. (2013) Scaffoldless tissue engineering: a model to study tissue formation and a device for tissue regeneration. Doctoral Dissertation, University of Pittsburgh. (Unpublished)

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Traditional tissue engineering methods involve the combination of cells, growth factors and a scaffold to regenerate 3D tissues for therapeutic purposes. Naturally, cells utilize their endogenous matrix as a scaffold to form 3D structures. The goals of this thesis were to study scaffoldless 3D constructs as a model of tissue formation and as a device for dental and bone regenerative therapies. Scaffoldless 3D constructs were first used as a model that showed that calcium phosphates are osteoinductive and alter 3D spatial differentiation patterns of dental pulp cells through modulations of connexin 43 expression. Scaffoldless 3D constructs were next used as a model system that showed tissues formed from an isolated population of dental pulp pericyte stem cells regenerated structurally and molecularly different 3D tissues than the total population of dental pulp cells. These studies emphasize the importance of scaffold and cell selection for regenerative therapies. Scaffoldless 3D dental pulp cell tissues were then used as a device that regenerated pulp-like tissue in the root canals of human tooth root segments. Dental pulp cells delivered into the root canals with calcium phosphate particles as a delivery system formed a mixture of dentin and pulp-like tissues. These two cell delivery systems show promise for various endodontic therapies. Finally, scaffoldless bone marrow stromal cell sheets were wrapped around calcium phosphate cements to create a structurally and molecularly relevant periosteum-like tissue that will aid in bone regeneration. These studies show the significance of using engineered tissues generated and organized entirely by the cells themselves and emphasize that scaffoldless engineered tissues are powerful tools for dental and bone research and regeneration.


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Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
Syed-Picard, Fatima N.fns4@pitt.eduFNS4
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairSfeir, Charlescsfeir@pitt.eduCSFEIR
Committee MemberBeniash, Eliaebeniash@pitt.eduEBENIASH
Committee MemberDavidson, Lance Alad43@pitt.eduLAD43
Committee MemberRobey,
Committee MemberWang, Yadongyaw20@pitt.eduYAW20
Date: 28 June 2013
Date Type: Publication
Defense Date: 27 November 2012
Approval Date: 28 June 2013
Submission Date: 12 November 2012
Access Restriction: 5 year -- Restrict access to University of Pittsburgh for a period of 5 years.
Number of Pages: 165
Institution: University of Pittsburgh
Schools and Programs: Swanson School of Engineering > Bioengineering
Degree: PhD - Doctor of Philosophy
Thesis Type: Doctoral Dissertation
Refereed: Yes
Uncontrolled Keywords: tooth, bone, stem cells, tissue engineering, scaffoldless, gap junction
Date Deposited: 28 Jun 2013 19:08
Last Modified: 28 Jun 2018 05:15


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