Lewis, JA and Hatfull, GF
(2000)
Identification and characterization of mycobacteriophage L5 excisionase.
Molecular Microbiology, 35 (2).
350 - 360.
ISSN 0950-382X
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Abstract
The well-characterized mycobacteriophage L5 forms stable lysogens in Mycobacterium smegmatis. Establishment of lysogeny involves integration of the phage genome into the chromosome of its mycobacterial hosts through an integrase-mediated site-specific recombination event. As L5 lysogens spontaneously generate free phage particles, prophage excision must also occur, although an L5 excisionase gene had not been identified. We show here that L5 gene 36 encodes the phage excisionase and is a small, heat-stable 56-amino-acid protein that strongly stimulates excisive recombination both in vivo and in vitro. The ability to manipulate the highly directional phage integration and excision reactions will provide powerful tools for the introduction, curing and recovery of foreign genes in recombinant mycobacterial strains.
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Details
Item Type: |
Article
|
Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
---|
Lewis, JA | | | | Hatfull, GF | gfh@pitt.edu | GFH | |
|
Date: |
31 January 2000 |
Date Type: |
Publication |
Journal or Publication Title: |
Molecular Microbiology |
Volume: |
35 |
Number: |
2 |
Page Range: |
350 - 360 |
DOI or Unique Handle: |
10.1046/j.1365-2958.2000.01695.x |
Schools and Programs: |
Dietrich School of Arts and Sciences > Biological Sciences |
Refereed: |
Yes |
ISSN: |
0950-382X |
MeSH Headings: |
Amino Acid Sequence; Attachment Sites, Microbiological--genetics; DNA Nucleotidyltransferases--chemistry; DNA Nucleotidyltransferases--genetics; DNA Nucleotidyltransferases--metabolism; DNA, Viral--analysis; DNA, Viral--genetics; Electrophoresis, Polyacrylamide Gel; Molecular Sequence Data; Mycobacteriophages--enzymology; Mycobacteriophages--genetics; Mycobacteriophages--physiology; Mycobacterium smegmatis--virology; Plasmids--genetics; Polymerase Chain Reaction; Recombination, Genetic; Sequence Alignment; Viral Proteins; Virus Integration |
PubMed ID: |
10652095 |
Date Deposited: |
29 Nov 2012 21:02 |
Last Modified: |
02 Feb 2019 15:57 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/16352 |
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