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Mycobacteriophage marvin: A new singleton phage with an unusual genome organization

Mageeney, C and Pope, WH and Harrison, M and Moran, D and Cross, T and Jacobs-Sera, D and Hendrix, RW and Dunbar, D and Hatfull, GF (2012) Mycobacteriophage marvin: A new singleton phage with an unusual genome organization. Journal of Virology, 86 (9). 4762 - 4775. ISSN 0022-538X

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Abstract

Mycobacteriophages represent a genetically diverse group of viruses that infect mycobacterial hosts. Although more than 80 genomes have been sequenced, these still poorly represent the likely diversity of the broader population of phages that can infect the host, Mycobacterium smegmatis mc2155. We describe here a newly discovered phage, Marvin, which is a singleton phage, having no previously identified close relatives. The 65,100-bp genome contains 107 predicted protein-coding genes arranged in a noncanonical genomic architecture in which a subset of the minor tail protein genes are displaced about 20 kbp from their typical location, situated among nonstructural genes anticipated to be expressed early in lytic growth. Marvin is not temperate, and stable lysogens cannot be recovered from infections, although the presence of a putative xis gene suggests that Marvin could be a relatively recent derivative of a temperate parent. The Marvin genome is replete with novel genes not present in other mycobacteriophage genomes, and although most are of unknown function, the presence of amidoligase and glutamine amidotransferase genes suggests intriguing possibilities for the interactions of Marvin with its mycobacterial hosts. © 2012, American Society for Microbiology.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Mageeney, C
Pope, WHwelkin@pitt.eduWELKIN0000-0002-2012-2924
Harrison, M
Moran, D
Cross, T
Jacobs-Sera, Ddjs@pitt.eduDJS0000-0001-7838-3257
Hendrix, RWrhx@pitt.eduRHX
Dunbar, D
Hatfull, GFgfh@pitt.eduGFH
Date: 1 May 2012
Date Type: Publication
Journal or Publication Title: Journal of Virology
Volume: 86
Number: 9
Page Range: 4762 - 4775
DOI or Unique Handle: 10.1128/jvi.00075-12
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0022-538X
MeSH Headings: DNA, Viral--chemistry; Gene Order; Genome, Viral; Molecular Sequence Annotation; Mycobacteriophages--genetics; Mycobacteriophages--isolation & purification; Mycobacteriophages--ultrastructure; Mycobacterium smegmatis--virology; Sequence Analysis, DNA; Viral Proteins--genetics; Viral Proteins--metabolism; Virion--chemistry; Virion--ultrastructure
Other ID: NLM PMC3347389
PubMed Central ID: PMC3347389
PubMed ID: 22357284
Date Deposited: 03 Dec 2012 22:53
Last Modified: 02 Feb 2019 15:57
URI: http://d-scholarship.pitt.edu/id/eprint/16355

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