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Genome organization and characterization of mycobacteriophage Bxb1

Mediavilla, J and Jain, S and Kriakov, J and Ford, ME and Duda, RL and Jacobs, WR and Hendrix, RW and Hatfull, GF (2000) Genome organization and characterization of mycobacteriophage Bxb1. Molecular Microbiology, 38 (5). 955 - 970. ISSN 0950-382X

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Mycobacteriophage Bxb1 is a temperate phage of Mycobacterium smegmatis. The morphology of Bxb1 particles is similar to that of mycobacteriophages L5 and D29, although Bxb1 differs from these phages in other respects. First, it is heteroimmune with L5 and efficiently forms plaques on an L5 lysogen. Secondly, it has a different host range and fails to infect slow-growing mycobacteria, using a receptor system that is apparently different from that of L5 and D29. Thirdly, it is the first mycobacteriophage to be described that forms a large prominent halo around plaques on a lawn of M. smegmatis. The sequence of the Bxb1 genome shows that it possesses a similar overall organization to the genomes of L5 and D29 and shares weak but detectable DNA sequence similarity to these phages within the structural genes. However, Bxb1 uses a different system of integration and excision, a repressor with different specificity to that of L5 and encodes a large number of novel gene products including several with enzymatic functions that could degrade or modify the mycobacterial cell wall.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Mediavilla, J
Jain, S
Kriakov, J
Ford, ME
Duda, RLduda@pitt.eduDUDA0000-0002-9517-2731
Jacobs, WR
Hendrix, RWrhx@pitt.eduRHX
Hatfull, GFgfh@pitt.eduGFH
Date: 1 December 2000
Date Type: Publication
Journal or Publication Title: Molecular Microbiology
Volume: 38
Number: 5
Page Range: 955 - 970
DOI or Unique Handle: 10.1046/j.1365-2958.2000.02183.x
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0950-382X
MeSH Headings: Amino Acid Sequence; Base Sequence; DNA, Viral; Genome, Viral; Molecular Sequence Data; Mycobacteriophages--genetics; Mycobacterium smegmatis--virology; Repressor Proteins--chemistry; Repressor Proteins--genetics; Viral Proteins--chemistry; Viral Proteins--genetics
PubMed ID: 11123671
Date Deposited: 03 Dec 2012 22:48
Last Modified: 02 Feb 2019 15:57


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