Mediavilla, J and Jain, S and Kriakov, J and Ford, ME and Duda, RL and Jacobs, WR and Hendrix, RW and Hatfull, GF
(2000)
Genome organization and characterization of mycobacteriophage Bxb1.
Molecular Microbiology, 38 (5).
955 - 970.
ISSN 0950-382X
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Abstract
Mycobacteriophage Bxb1 is a temperate phage of Mycobacterium smegmatis. The morphology of Bxb1 particles is similar to that of mycobacteriophages L5 and D29, although Bxb1 differs from these phages in other respects. First, it is heteroimmune with L5 and efficiently forms plaques on an L5 lysogen. Secondly, it has a different host range and fails to infect slow-growing mycobacteria, using a receptor system that is apparently different from that of L5 and D29. Thirdly, it is the first mycobacteriophage to be described that forms a large prominent halo around plaques on a lawn of M. smegmatis. The sequence of the Bxb1 genome shows that it possesses a similar overall organization to the genomes of L5 and D29 and shares weak but detectable DNA sequence similarity to these phages within the structural genes. However, Bxb1 uses a different system of integration and excision, a repressor with different specificity to that of L5 and encodes a large number of novel gene products including several with enzymatic functions that could degrade or modify the mycobacterial cell wall.
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Details
| Item Type: |
Article
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| Status: |
Published |
| Creators/Authors: |
|
| Date: |
1 December 2000 |
| Date Type: |
Publication |
| Journal or Publication Title: |
Molecular Microbiology |
| Volume: |
38 |
| Number: |
5 |
| Page Range: |
955 - 970 |
| DOI or Unique Handle: |
10.1046/j.1365-2958.2000.02183.x |
| Schools and Programs: |
Dietrich School of Arts and Sciences > Biological Sciences |
| Refereed: |
Yes |
| ISSN: |
0950-382X |
| MeSH Headings: |
Amino Acid Sequence; Base Sequence; DNA, Viral; Genome, Viral; Molecular Sequence Data; Mycobacteriophages--genetics; Mycobacterium smegmatis--virology; Repressor Proteins--chemistry; Repressor Proteins--genetics; Viral Proteins--chemistry; Viral Proteins--genetics |
| PubMed ID: |
11123671 |
| Date Deposited: |
03 Dec 2012 22:48 |
| Last Modified: |
02 Feb 2019 15:57 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/16363 |
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